LCAT acts as a molecular switch and regulates rooster sperm motility in vitro.

Jiwei Li, Yuzhu Cao, Juan Zhang, Yadong Tian, Guirong Sun, Yaling Gu, Xiangtao Kang, Donghua Li

Abstract read

In one paragraph

Article in Molecular & cellular proteomics : MCP, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Jiwei LiHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China; College of Animal Science and Technology, Ningxia University, Yinchuan, China.

Yuzhu CaoHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.

Juan ZhangCollege of Animal Science and Technology, Ningxia University, Yinchuan, China.

Yadong TianHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.

Guirong SunHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.

Yaling GuCollege of Animal Science and Technology, Ningxia University, Yinchuan, China. Electronic address: guyl@nxu.edu.cn.

Xiangtao KangHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China. Electronic address: xtkang2001@263.net.

Donghua LiHenan Key Laboratory for Innovation and Utilization of Chicken Germplasm Resources, College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China. Electronic address: lidonghua6656@henau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sperm motility is a critical determinant of semen quality, yet the molecular mechanisms regulating motility remain unclear. In this study, we identified Yufen 1 roosters with significant differences in sperm motility among highly motile sperm samples stored under different conditions. By integrating the seminal plasma proteome and seminal plasma metabolome data, 181 proteins and 112 metabolites were identified as differentially expressed between the high motility group (HMG) and low motility group (LMG). Lecithin cholesterol acyltransferase (LCAT) was upregulated in the LMG. Targeted LCAT activation significantly reduced sperm motility and plasma membrane integrity, caused sperm plasma membrane disruption, chromatin loss, and mitochondrial swelling, and reduced the longest fertilization days and number of fertilized eggs. Mechanistically, LCAT regulates sperm motility in vitro by altering sperm membrane structure and function by modulating glycerophospholipid metabolism. These findings provide new insights into extending the window period for artificial insemination and semen preservation strategies in poultry.

Indexed as

ChickensSpermatozoaSperm MotilityAnimalsCell MembraneGlycerophospholipidsMaleProteomeSemenGlycerophospholipidsProteomeglycerophospholipid metabolismLCATmaintenance of vitalityseminal plasmasperm motility

Identifiers

PMID42242641

DOI10.1016/j.mcpro.2026.101598

PMCPMC13330670

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.