Evidence map›Paper›PMID 42242218›Full record

ArticleNeuron2026

POINTseq: Cell-type-specific barcoding reveals single-cell projection architecture of the mouse dopaminergic system.

Hyopil Kim, Cheng Xu, Craig Washington, Caleb Shi, Maggie Lowman, Justus M Kebschull

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Hyopil KimDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Cheng XuDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Craig WashingtonDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Caleb ShiDepartment of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA.
Maggie LowmanDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Justus M KebschullDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA; Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA; Center for Functional Anatomy and Evolution, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: kebschull@jhu.edu.

Funding

BRAIN CONNECTS: Mapping brain-wide connectivity of neuronal types using barcoded connectomicsU01NS132161 · NINDS · ALLEN INSTITUTE · PI CHEN, XIAOYIN, KEBSCHULL, JUSTUS M · 2023 to 2025
$6.7M
Cell type specific vulnerability to agingRF1AG078378 · NIA · JOHNS HOPKINS UNIVERSITY · PI PATRICK O KANOLD, JUSTUS M KEBSCHULL · 2023 to 2026
$4.0M
Circuitry dynamics underlying opioid-dependence: Integrating structural, functional, and transcriptomic mechanismsR01DA056599 · NIDA · UNIVERSITY OF PENNSYLVANIA · PI KEVIN T BEIER, Julie A Blendy · 2022 to 2026
$3.8M
IDENTIFYING EPIGENOMICS AND CONNECTOMICS OF PROTRACTED OPIOID WITHDRAWAL AND RELAPSE USING CELLULAR BARCODINGDP1DA056668 · NIDA · JOHNS HOPKINS UNIVERSITY · PI JUSTUS M KEBSCHULL · 2022 to 2026
$2.5M
Mapping opioid-dependence state transitions across structural, functional, and transcriptomic topologiesR01DA054374 · NIDA · UNIVERSITY OF PENNSYLVANIA · PI BLENDY, JULIE A, CORDER, GREGORY · 2021 to 2025
$2.4M
Integrating single-cell connectivity, gene expression, and function in zebra finchesR34NS132027 · NINDS · JOHNS HOPKINS UNIVERSITY · PI KEBSCHULL, JUSTUS M · 2023 to 2024
$721k
NIA NIH HHS RF1 AG078378NIDA NIH HHS DP1 DA056668NIDA NIH HHS R01 DA054374NIDA NIH HHS R01 DA056599NINDS NIH HHS R34 NS132027NINDS NIH HHS U01 NS132161
6 · The paper itself

Abstract

Neural circuits are shaped by the diverse axonal branching patterns of neurons across different cell types. To map these patterns, here we introduce POINTseq (projections of interest by sequencing), a barcoded connectomics method for rapid, cell-type-specific mapping of thousands of single-cell projections per animal. POINTseq leverages viral pseudotyping and cell-type-specific infection to integrate MAPseq-style high-throughput barcoded projection mapping with the established viral-genetic neural circuit analysis toolbox. We validated POINTseq by mapping genetically and projection-defined cell populations in the mouse motor cortex. We then used POINTseq to reconstruct the brain-wide projections of 5,902 individual dopaminergic neurons in the ventral tegmental area (VTA) and substantia nigra pars compacta (SNc). These neurons fall into >25 connectomic cell types, vastly exceeding the known diversity of dopaminergic cells, and form stereotyped projection motifs that may mediate parallel dopamine signaling. These data constitute the anatomical substrate on which the diverse functions of dopamine in the brain are built.

Indexed as

barcodingcell typesconnectomicsdopamineMAPseqmidbrainpseudotyped virussingle-cell projectionsSNcVTA

Identifiers

PMID42242218
PMCPMC13245377

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.