ArticleSTAR protocols2026
Protocol for detecting protein S-glutathionylation in Arabidopsis thaliana under oxidative stress using a non-transgenic chemical toolkit.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Protein S-glutathionylation is a redox-sensitive post-translational modification that regulates proteostasis and signaling during oxidative stress. However, its in vivo detection is challenging due to labile disulfide bonds and ex vivo oxidation artifacts. Here, we present a protocol for detecting protein glutathionylation in Arabidopsis thaliana using a non-transgenic toolkit. We describe steps for using propargyl glycine (PG) metabolic labeling and click chemistry. We detail workflows for global quantification, target enrichment for western blotting or liquid chromatography-tandem mass spectrometry (LC-MS/MS), and in situ spatial visualization. For complete details on the development, use and execution of this protocol, please refer to Zhao et al.
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