Evidence map›Paper›PMID 42240781›Full record

ArticleMarine biotechnology (New York, N.Y.)2026

Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus.

Fengyi Liu, Qilin Geng, Xue Sun, Jie Lin, Xuan Zhu, Danyang Wang, Xiao-Hua Zhang, Jingjie Hu, Shi Wang, Lisui Bao and 1 more

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Fengyi Liu *Fang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Qilin Geng *Fang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Xue SunFang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Jie LinFang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Xuan ZhuFang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Danyang WangSouthern Marine Science and Engineering Guangdong Laboratory, Guangzhou, China.
Xiao-Hua ZhangFrontiers Science Center for Deep Ocean Multispheres and Earth System, College of Marine Life Sciences, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, 266003, China.
Jingjie HuFang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Shi WangFang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Lisui BaoFang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. baolisui@ouc.edu.cn.
Jia LvFang Zongxi Center for Marine Evo-Devo & MOE Key Laboratory of Marine Genetics and Breeding & Qingdao Institute of Blue Seed Industry, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China. lvjia@ouc.edu.cn.

Funding

Blue Seed Industry Science and Technology Innovation Project QDLYY-2024011Fundamental Research Funds for the Central Universities 202562018Major Program for the Fundamental Research of the Natural Science Foundation of Shandong Province ZR2025ZD06Marine S&T Fund of Shandong Province for Laoshan Laboratory LSKJ202202803National Natural Science Foundation of China 32573498Shandong Provincial Natural Science Fund for Excellent Young Scientists Fund Program (Overseas) 2023HWYQ-063
6 · The paper itself

Abstract

Vibrio parahaemolyticus (V. parahaemolyticus), a significant pathogen in aquatic products, necessitates rapid and precise detection to prevent foodborne disease outbreaks. Early detection of bacterial pathogens is crucial for effective prevention and control. In this study, we developed a field-deployable, "sample-in, answer-out" workflow that integrates efficient DNA extraction with visual fluorescence loop-mediated isothermal amplification (LAMP) for the on-site detection of V. parahaemolyticus. This extraction protocol enables efficient one-pot DNA release within 10 min, eliminating the need for additional purification steps. In addition, coupled with an optimized fluorescence LAMP assay targeting V. parahaemolyticus, the entire process-from sample to result-can be completed within approximately 50 min. The optimized assay exhibited high sensitivity, detecting as low as 1 copy/µL of plasmid DNA and 10 CFU per reaction from pure bacterial culture and spiked seawater samples. This extraction-amplification integrated approach demonstrates robust performance and high practicality for on-site early warning and pathogen monitoring in aquaculture safety settings.

Indexed as

DNA, BacterialNucleic Acid Amplification TechniquesVibrio parahaemolyticusAnimalsAquacultureFluorescenceMolecular Diagnostic TechniquesSeawaterSensitivity and SpecificityVibrio InfectionsDNA, BacterialExtraction-amplification integrated approachRapid detectionSample-in, answer-outVibrio parahaemolyticus

Identifiers

PMID42240781

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.