Evidence map›Paper›PMID 42240679›Full record

ArticleActa crystallographica. Section F, Structural biology communications2026

Crystal structure of the cytosolic Nudix-like domain of CD-NTase-associated protein 16 from Enterococcus faecalis.

Tinashe Makanyire, Zihan Zhou, Wanqian Wu, Mingyan Li, Shenglan Xia, HaoYuan Shi, Jianping Kong, Zhaoxing Li, Yibei Xiao

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In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Tinashe MakanyireState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
Zihan ZhouState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
Wanqian WuState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
Mingyan LiSchool of Science, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
Shenglan XiaState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
HaoYuan ShiState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
Jianping KongState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
Zhaoxing LiState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.
Yibei XiaoState Key Laboratory of Natural Medicines, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, People's Republic of China.

Funding

China Pharmaceutical University SKLNMZZ202508Ministry of Education of the People's Republic of China JYB2025XDXM601National Natural Science Foundation of China 82304614National Natural Science Foundation of China 82321005National Natural Science Foundation of China 82473977National Science and Technology Major Project 2021ZD0203400Natural Science Foundation of Jiangsu Province SBK2024010634
6 · The paper itself

Abstract

The cyclic oligonucleotide-based antiphage signaling system (CBASS) employs diverse effector proteins to trigger abortive infection upon phage invasion, yet structural information on membrane-associated CBASS effectors remains limited. CD-NTase-associated protein 16 from Enterococcus faecalis (EfCap16) is a predicted CBASS effector comprising an N-terminal transmembrane region and a C-terminal Nudix-like domain. Here, we report the recombinant expression, purification, crystallization and X-ray crystal structure of the cytosolic Nudix domain of EfCap16 (PDB entry 22lg) in space group P12

Indexed as

Bacterial ProteinsCytosolEnterococcus faecalisPyrophosphatasesAmino Acid SequenceCloning, MolecularCrystallizationCrystallography, X-RayEscherichia coliGene ExpressionModels, MolecularNudix HydrolasesProtein DomainsRecombinant ProteinsBacterial ProteinsNudix HydrolasesPyrophosphatasesRecombinant ProteinsCBASSNudix hydrolasestransmembrane effectors

Identifiers

PMID42240679
PMCPMC13335047

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.