ArticleJournal of clinical laboratory analysis2026
Evaluation of the Application of Urea Dissociation Treatment to Reduce the False-Positive Rate of ELISA for Syphilis Detection.
Article in Journal of clinical laboratory analysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveExploring the application of urea dissociation treatment to reduce the false-positive rate of ELISA for syphilis detection.
methodsFrom January 2023 to January 2024, eleven false-positive syphilis samples were collected from Xi'an Regional Medical Laboratory Centre as the experimental group, five positive specimens as the control group, and five negative specimens as the control group. All specimens were divided into three groups: A, B, and C. They were treated with 6 mol/L urea for 10 min, 6 mol/L urea for 20 min, and 10 mol/L urea for 10 min, respectively, and then processed according to the experimental procedures. Combined with the analysis of the TPPA and TP immunoblotting results, the Wilcoxon test was used to compare the S/CO values of each group.
resultsSpecimens 2 and 3 still had no < 1.0 S/CO in group A, while B and C had < 1.0 S/CO after completion of the separation. Meanwhile, specimens 7 and 8 of the experimental group had S/CO close to 1.0 after dissociation in all three groups. The positive control group had an S/CO ratio of greater than 1.0 in all three groups. The negative control specimens, both before and after separation, had no effect. The differences in S/CO values between experimental groups A, B, and C before and after dissociation were statistically significant (p < 0.05). The values of TP are below the manufacturer's allowable imprecision requirements.
conclusionUrea dissociation by ELISA is effective in reducing the false-positive rate of syphilis.
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