Evidence map›Paper›PMID 42239444›Full record

ArticlebioRxiv : the preprint server for biology2026

Mitotic kinase regulation of DNA replication forks.

Sudikchya Shrestha, Natasia Paukovich, Samuel R Greenfield, Andrea MacFadden, Shania N Smith, Shaun Bevers, Allison W McClure

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sudikchya ShresthaDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz, Aurora, CO, USA.ORCID 0000-0002-2790-7804
Natasia PaukovichDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz, Aurora, CO, USA.ORCID 0000-0002-8916-9122
Samuel R GreenfieldDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz, Aurora, CO, USA.ORCID 0009-0005-3414-0866
Andrea MacFaddenDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz, Aurora, CO, USA.ORCID 0000-0002-5960-6109
Shania N SmithDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz, Aurora, CO, USA.ORCID 0009-0006-2629-9276
Shaun BeversDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz, Aurora, CO, USA.ORCID 0000-0002-2000-6106
Allison W McClureDepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz, Aurora, CO, USA.ORCID 0000-0002-5321-2483

Funding

Predoctoral Training Program in Molecular and Cellular Biology (Supplement: Mentoring in the Research Environment)T32GM136444 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI MICHAEL A MCMURRAY, Rytis Prekeris · 2020 to 2026
$3.7M
Molecular mechanisms of DNA replication in mitosisR35GM156773 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI Allison McClure · 2025 to 2026
$780k
NIGMS NIH HHS R35 GM156773NIGMS NIH HHS T32 GM136444
6 · The paper itself

Abstract

While DNA replication forks initiate in S phase, they do not necessarily complete and terminate prior to cell entry into mitosis. How mitotic proteins regulate leftover replication forks is not well-understood. Using reconstituted DNA replication forks with purified proteins, we show that the budding yeast mitotic kinases Clb2-CDK (M-CDK) and Cdc5 (Plk1 homolog) phosphorylate and regulate several replication elongation proteins. Mrc1 phosphorylation by both kinases results in slower replication, and Polα phosphorylation by M-CDK results in less lagging strand initiation. We further show that a phospho-resistant mutant of Polα bypasses M-CDK inhibition of Polα activity in reconstituted replication reactions. Yeast cells expressing the phospho-resistant mutant exhibit faster cell cycle progression revealing a potential negative feedback mechanism between DNA replication forks and mitotic progression.

Identifiers

PMID42239444
PMCPMC13228482

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.