ArticlebioRxiv : the preprint server for biology2026
Dissecting the role of PCNA and Pif1 in replication of individual DNA molecules by DNA polymerase δ.
Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
PCNA loading and stabilization at primer-template junctions is crucial for processive DNA synthesis by replicative polymerases. It is also essential for strand displacement synthesis during Break Induced Replication (BIR). In this study, we employed a single molecule approach to directly visualize the role of PCNA, DNA polymerase δ and the Pif1 DNA helicase in these pathways. We first dissected the preferential loading of PCNA by RFC to 3'-junctions of gaps, nicks and flapped substrates. Moreover, we found that Pol δ and Pif1 both stabilize loaded PCNA and reduce its diffusion coefficient along DNA. We further show that the interaction of PCNA with Pol δ is essential for gap-filling synthesis. On a 5'-flapped substrate, we show that both Pol δ and Pif1 are required for strand displacement synthesis, while PCNA is not; although, it stimulates the process.
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