Evidence map›Paper›PMID 42239221›Full record

ArticlebioRxiv : the preprint server for biology2026

Single amino acid substitution in DNA Polymerase I dramatically alters infection dynamics of bacteriophage T7.

Rachel A Keown, Andrew P Sikkema, Victoria A Barbone, Barbra D Ferrell, Owen B Donnelly, Sydney C Iredell, Kelly M Zatopek, Phillip J Brumm, David A Mead, Gregory J S Lohman and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Rachel A KeownCenter for Bioinformatics and Computational Biology, Department of Computer and Information Sciences, University of Delaware, Newark, DE 19713, USA.ORCID 0000-0002-5631-8689
Andrew P SikkemaApplied Molecular Biology Division, Research Department, New England Biolabs, Ipswich, MA 01938, USA.ORCID 0000-0003-0968-1845
Victoria A BarboneDepartment of Medical and Molecular Sciences, University of Delaware, Newark, DE 19713, USA.
Barbra D FerrellDepartment of Plant and Soil Sciences, University of Delaware, Newark, DE 19713, USA.ORCID 0000-0003-3057-3478
Owen B DonnellyDepartment of Biological Sciences, University of Delaware, Newark, DE 19713, USA.
Sydney C IredellDepartment of Animal and Food Sciences, University of Delaware, Newark, DE 19713, USA.
Kelly M ZatopekApplied Molecular Biology Division, Research Department, New England Biolabs, Ipswich, MA 01938, USA.ORCID 0000-0002-0787-8495
Phillip J BrummVarizymes, Middleton, WI 52562, USA.
David A MeadVarizymes, Middleton, WI 52562, USA.ORCID 0000-0003-1982-9356
Gregory J S LohmanApplied Molecular Biology Division, Research Department, New England Biolabs, Ipswich, MA 01938, USA.ORCID 0000-0002-0638-7555
K Eric WommackDepartment of Plant and Soil Sciences, University of Delaware, Newark, DE 19713, USA.ORCID 0000-0001-5797-7128
Shawn W PolsonCenter for Bioinformatics and Computational Biology, Department of Computer and Information Sciences, University of Delaware, Newark, DE 19713, USA.ORCID 0000-0002-3398-6932

Funding

Predictive Modeling & Optimal Control Framework for Model-Based Epidemic Response in DelawareP20GM103446 · NIGMS · UNIVERSITY OF DELAWARE · PI Shawn W Polson · 2012 to 2026
$67.2M
Practical Data-Centric AI/ML for Biomedical ResearchersT32GM142603 · NIGMS · UNIVERSITY OF DELAWARE · PI Shawn W Polson, Abhyudai Singh · 2022 to 2026
$1.4M
Data Management and Storage System for Shared Resource FacilitiesS10OD028725 · OD · UNIVERSITY OF DELAWARE · PI POLSON, SHAWN W · 2021 to 2021
$600k
NIGMS NIH HHS P20 GM103446NIGMS NIH HHS T32 GM142603NIH HHS S10 OD028725
6 · The paper itself

Abstract

Viruses constitute a significant proportion of Earth's genetic diversity, yet most remain uncharacterized beyond their sequences in viral metagenomes. Linking viral genotypes to phenotypes-especially enzyme function to phage infection dynamics-is challenging due to the lack of cultured virus-host systems. DNA polymerase I (PolA), essential for genome replication in ~25% of dsDNA phages, provides an opportunity to explore these connections. In phage T7, residue 526 is critical for nucleotide incorporation, with previous

Identifiers

PMID42239221
PMCPMC13228545

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.