Evidence map›Paper›PMID 42239209›Full record

ArticlebioRxiv : the preprint server for biology2026

Ligation-assisted target recycling for DNA nanoswitch biosensors.

Vinod Morya, Andrew Hayden, Mona Zeghal, Jibin Abraham Punnoose, Ken Halvorsen

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Vinod MoryaThe RNA Institute, University at Albany, State University of New York, Albany, NY, 12222 USA.ORCID 0000-0002-1539-4108
Andrew HaydenThe RNA Institute, University at Albany, State University of New York, Albany, NY, 12222 USA.
Mona ZeghalThe RNA Institute, University at Albany, State University of New York, Albany, NY, 12222 USA.
Jibin Abraham PunnooseThe RNA Institute, University at Albany, State University of New York, Albany, NY, 12222 USA.ORCID 0000-0003-2367-6874
Ken HalvorsenThe RNA Institute, University at Albany, State University of New York, Albany, NY, 12222 USA.ORCID 0000-0002-2578-1339

Funding

Manipulating nucleic acids: applications in RNA biosensing, single-molecule analysis, and DNA nanotechnologyR35GM124720 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Ken A Halvorsen · 2017 to 2026
$4.6M
NIGMS NIH HHS R35 GM124720
6 · The paper itself

Abstract

Conformationally responsive DNA nanoswitches have previously been developed and validated for a variety of biosensing applications including detection of DNA, microRNA, and viral RNA/DNA. Here we develop new methodology for enhancing the sensitivity of DNA-based sensing by recycling a fixed number of targets for repeated reuse. We achieved target-dependent enzymatic ligation of looped nanoswitches and showed that subsequent removal of target does not affect the ligated loop. Through cyclic annealing, ligation, and target removal, we can linearly control signal amplification up to hundreds of cycles. This method adds an important new capability for low abundance targets without the need for target amplification.

Identifiers

PMID42239209
PMCPMC13228399

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.