Evidence map›Paper›PMID 42238519›Full record

ArticleSynthetic and systems biotechnology2026

Directed evolution of heme biosynthetic enzymes enabled by a growth-coupled heme biosensor in

Fangming Mou, Dong Li, Hong Wang, Jianbo Li, Tao Chen, Zhiwen Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Synthetic and systems biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Fangming MouState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, 300072, China.
Dong LiSchool of Life Sciences, Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan, 750021, China.
Hong WangState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, 300072, China.
Jianbo LiState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, 300072, China.
Tao ChenState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, 300072, China.
Zhiwen WangState Key Laboratory of Synthetic Biology, Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin, 300072, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heme is an essential cofactor involved in diverse cellular processes and is an important target for microbial biosynthesis. However, engineering microbial heme production remains challenging due to the requirement for coordinated activity of multiple pathway enzymes and the lack of scalable strategies for enzyme-level screening. In this study, we developed a growth-coupled screening system in

Indexed as

ChrSA two-component systemCorynebacterium glutamicumDirected evolutionGrowth-coupled biosensorHeme biosynthesis

Identifiers

PMID42238519
PMCPMC13226126

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.