ArticleThe plant pathology journal2026
Chitosan-Tripolyphosphate Nanoparticles Enhance Foliar dsRNA Delivery and Suppress Turnip Crinkle Virus in Arabidopsis.
Article in The plant pathology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Topically applied double-stranded RNA (dsRNA) can trigger antiviral RNA interference in plants, but naked dsRNA is unstable and poorly taken up after foliar application. Here, we developed an eco-friendly chitosan- tripolyphosphate (CS-TPP) nanocarrier to enhance dsRNA delivery using turnip crinkle virus (TCV) as a model and tested it in Arabidopsis thaliana. A dsRNA targeting the TCV-coat protein (CP) gene was produced in Escherichia coli, and complexation with CS-TPP was induced by ionic gelation. Complete complexation occurred at a 1:1 CS-TPP:dsRNA ratio, producing cationic nanoparticles that protected dsRNA from RNase digestion and maintained its integrity during incubation at 37°C. Using Cy3 fluorescence imaging, we observed stronger epidermis-associated fluorescence in plants treated with the CS-TPP@dsRNA complex than in those treated with naked dsRNA, and RT-qPCR confirmed higher CP-target RNA fragment accumulation in planta. Small RNA sequencing revealed increased levels of TCV-derived, 21-24 nt siRNA molecules targeting the CP region, suggesting the applied dsRNA was processed in planta. Importantly, a CS-TPP@ TCV-dsRNA foliar spray application reduced systemic CP RNA levels at 7 and 14 days post-treatment and alleviated disease symptoms more effectively than naked dsRNA. These results demonstrate that CS-TPP nanocomplexation improves dsRNA stability and delivery, enabling improved and more durable antiviral protection.
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