Evidence map›Paper›PMID 42237109›Full record

ArticleBMC plant biology2026

Multi-model GWAS reveals key loci for leaf angle in maize inbred lines from southeast China.

Keyu Li, Jia Xie, Jinghan Cao, Changjin Wang, Wangfei He, Renjie She, Ruzhen Bao, Yuchen Chai, Haoze Yu, Yu Han and 5 more

Abstract read
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

15 authors.

Keyu LiCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Jia XieCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Jinghan CaoCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Changjin WangCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Wangfei HeSuixi County Agricultural Research Experimental Station, Huaibei, 235000, China.
Renjie SheCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Ruzhen BaoCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Yuchen ChaiCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Haoze YuCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Yu HanCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Weidong HuangCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Xinxin ChengCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China.
Mingliang CuiSDIC Fengle Seed Co., Ltd, Hefei, 230000, China.
Lei ChenCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China. chenlei@ahstu.edu.cn.
Haibing YuCollege of Agriculture, Maize Germplasm Repository of Anhui Province, Anhui Science and Technology University, Chuzhou, 233100, China. yuhb@ahstu.edu.cn.

Funding

Science and Technology Innovation Groups of Anhui Science and Technology University 2023KJCXTD002the Anhui Provincial Major Science and Technology Project 2022e03020008the Key Discipline Construction Funds for Crop Science of Anhui Sciences and Technology University No. XK-XJGF001the Project No.2025SWYZ0200 supported by Bio-breeding Laboratory of Anhui province 2025SWYZ0200the research and development fund of Anhui Science and Technology University FZ230121the Talent Introduction Project of Anhui Science and Technology University NXYJ201901
6 · The paper itself

Abstract

backgroundMaize is a vital grain and forage crop with continuously increasing yield demands, making the improvement of plant architecture for high-density planting particularly significant. Among architectural traits, leaf angle (LA) is a key factor influencing light interception and canopy architecture. To dissect the genetic basis of LA in maize, a genome-wide association study was conducted.

resultsA panel of 212 maize inbred lines from breeding programs in Southeast China was genotyped using the Maize6H-60 K SNP array. Employing five GWAS models (GLM, MLM, SUPER, FarmCPU, and BLINK), we identified 14 significant SNPs (p < 8.55 × 10⁻⁷) and 342 candidate genes within the 200 kb flanking regions, 134 of which were functionally annotated. Multi-model cross-validation revealed six stably associated SNPs, including four novel loci (AX-107936049, AX-108102817, AX-107953621, and AX-108061477). AX-86,305,869 was located within 2 Mb of a previously reported significant SNP, while AX-107,994,145 fell within a known QTL interval. Within these six stable loci, 11 high-confidence candidate genes are putatively associated with LA variation, potentially acting via hormone signaling, leaf morphogenesis, and midvein development. Notably, GRMZM2G036905 (homologous to Arabidopsis ELF1) may play a role in regulating upright leaf architecture, and GRMZM2G003565 may contribute to leaf rolling as previously suggested. Based on these stable SNPs, KASP markers were developed and validated in 52 varieties/lines (including Zhengdan 958, MY73, and Anke 985) and 20 inbred lines, confirming the association between these SNPs and LA variation.

conclusionOur study identified six stable SNPs and 11 candidate genes for maize LA through multi-model GWAS in 212 inbred lines. Four novel loci were discovered, and KASP markers were developed for three stable SNPs, providing genetic resources and tools for breeding maize varieties tolerant to high planting density.

Indexed as

Genome-Wide Association StudyPlant LeavesQuantitative Trait LociZea maysChinaGenotypePhenotypePolymorphism, Single NucleotideCandidate genesGenome-wide association studiesInbred germplasmKASP markersLeaf angleMaize

Identifiers

PMID42237109
PMCPMC13450526

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