Evidence map›Paper›PMID 42236530›Full record

ArticleScientific reports2026

Identification and validation of reference genes for quantitative real-time PCR in Corydalis saxicola under various abiotic stresses.

Liang Kang, Zhaodi Wen, Han Liu, Ying Lu, Mei Qin, Lirong Huang, Ming Lei, Cui Li, Dan Zhu, Li Li and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Liang KangGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Zhaodi WenGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Han LiuGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Ying LuGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Mei QinGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Lirong HuangGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Ming LeiGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Cui LiGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China.
Dan ZhuSchool of Pharmacy, Guangxi Medical University, Nanning, 530021, China. zhudan@stu.gxmu.edu.cn.
Li LiSchool of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China. lily7819@whpu.edu.cn.
Zhanjiang ZhangGuangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory of Conservation and Genetic Improvement of Medicinal Plant Resources, Nanning, 530023, China. zzj1811@163.com.

Funding

Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement KL2025ZZ02; KL2023KF04Guangxi Key R&D Program Guike AB23026092Guangxi Qihuang Scholars Training Program GXQH202402Guangxi Zhuang Autonomous Region Traditional Chinese Medicine Administration Self-raised Funds Scientific Research Project GXZYA20250004the Central Guidance on Local Science and Technology Development Fund of Guangxi GK ZY24212031the National Natural Science Foundation of China 32460105; 32560103the Natural Science Foundation of Guangxi 2023GXNSFAA026509; 2025GXNSFBA069023; 2025GXNSFAA069814
6 · The paper itself

Abstract

Quantitative real-time PCR (qRT-PCR) is a commonly used method for measuring gene expression, but its accuracy depends on the use of stable reference genes for data normalization. In this study, we evaluated the expression stability of 11 candidate reference genes in Corydalis saxicola across different tissues and under three abiotic stress conditions (Ca²

Indexed as

CorydalisGenes, PlantReal-Time Polymerase Chain ReactionStress, PhysiologicalGene Expression ProfilingGene Expression Regulation, PlantReference StandardsAbiotic stressesCorydalis saxicolaGene expressionReal-time quantitative PCRReference genes

Identifiers

PMID42236530
PMCPMC13473475

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.