Evidence map›Paper›PMID 42236473›Full record

ArticleMicrosystems & nanoengineering2026

A smartphone-powered decentralized microfluidic platform for sensitive CRISPR-based nucleic acid detection.

Peng Zhou, Amber N McElroy, Yingming Xu, Andrew Decker, Timothy W Schacker, Terrence W Simon, Mark J Osborn, Tianhong Cui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microsystems & nanoengineering, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Peng Zhou *University of Minnesota, Minneapolis, MN, USA.ORCID http://orcid.org/0009-0003-0477-1566
Amber N McElroy *University of Minnesota, Minneapolis, MN, USA.
Yingming XuUniversity of Minnesota, Minneapolis, MN, USA.ORCID http://orcid.org/0009-0008-1284-5904
Andrew DeckerUniversity of Minnesota, Minneapolis, MN, USA.
Timothy W SchackerUniversity of Minnesota, Minneapolis, MN, USA.
Terrence W SimonUniversity of Minnesota, Minneapolis, MN, USA.
Mark J Osborn *University of Minnesota, Minneapolis, MN, USA. osbor026@umn.edu.
Tianhong Cui *University of Minnesota, Minneapolis, MN, USA. cuixx006@umn.edu.

Funding

Skin-targeted Cell Therapy for Recessive Dystrophic Epidermolysis BullosaR01AR063070 · NIAMS · UNIVERSITY OF MINNESOTA · PI Mark J Osborn, Jakub Tolar · 2013 to 2026
$4.5M
NIAMS NIH HHS R01 AR063070
6 · The paper itself

Abstract

Sensitive, portable, and low-cost nucleic acid detection without need for complicated equipment or trained operators, allows flexibility in forward (i.e., near the point of collection) detection and diagnostics that could be applied in resource-limited settings. To this end, we developed a microfluidic, chip-based laser-induced graphene (LIG) heater with precise temperature control for loop-mediated isothermal amplification (LAMP) of DNA or RNA and subsequent CRISPR/Cas9-based molecular interrogation. Detection was pursued using commercial lateral flow assays (LFAs) and an ultrasensitive electrochemical module that utilizes Hexaammineruthenium (III) chloride (RuHex) as a redox probe. Using LIG-based LAMP amplification we were able to amplify and detect targets in samples with as low as 5 copies per 10 μL. We also evaluated post amplification products to determine the threshold amount of analyte required to enable detection. Our device demonstrated detection limits of 10

Identifiers

PMID42236473
PMCPMC13234344

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.