Evidence map›Paper›PMID 42236213›Full record

ArticleThe Journal of veterinary medical science2026

Improvement of PCR primers for detection of Mycoplasma mycoides subsp. mycoides, the causative agent of contagious bovine pleuropneumonia.

Akari Tachibana, Rana Okawaki, Eiji Hata, Takashi Mada, Daisuke Takamatsu, Mariko Okamoto

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Akari TachibanaDepartment of Bacterial Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Rana OkawakiSeibu Livestock Hygiene Service Center, Tokushima, Japan.
Eiji HataDepartment of Transboundary Animal Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, Kagoshima, Japan.
Takashi MadaDepartment of Bacterial Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Daisuke TakamatsuDepartment of Bacterial Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Mariko OkamotoDepartment of Bacterial Diseases, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Contagious bovine pleuropneumonia (CBPP) is a severe respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides (Mmm). A method combining PCR and restriction enzyme analysis (PCR-REA) is described in the World Organisation for Animal Health manual to differentiate Mmm from the related species Mycoplasma mycoides subsp. capri. However, the primers in the manual did not amplify the target region from an attenuated Mmm strain. Genome sequence comparison revealed mismatches between the primers and the target sequences of Mmm strains. Newly designed primers with corrected bases successfully amplified the target region from M. mycoides strains, and REA patterns distinguished the two subspecies. These results indicate that the new primers provide reliable Mmm detection and enhance preparedness for future CBPP outbreaks.

Indexed as

Cattle DiseasesDNA PrimersMycoplasmaMycoplasma mycoidesPleuropneumonia, ContagiousPolymerase Chain ReactionAnimalsCattleDNA Primerscontagious bovine pleuropneumoniadiagnosisMycoplasma mycoides subsp. capriMycoplasma mycoides subsp. mycoidesPCR-restriction enzyme analysis

Identifiers

PMID42236213
PMCPMC13500397

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.