Evidence map›Paper›PMID 42234957›Full record

ArticleACS synthetic biology2026

Ancestral Protein Reconstruction Uncovers a Thermotolerant Rieske Oxygenase with Enhanced

Augusto Rodrigues Lima, Gabriel Gonçalves Dias, Samuel J Davis, Gabriela de Lima Menezes, Adriano Freitas Lima, Mariany da Silva Costa, Lúcia Daniela Wolf, Fernanda Miyuki Kashiwagi, Mariana Abrahão Bueno Morais, Mikael Bodén and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Augusto Rodrigues LimaBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.
Gabriel Gonçalves DiasBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.
Samuel J DavisSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
Gabriela de Lima MenezesBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.ORCID 0000-0002-8944-8148
Adriano Freitas LimaBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.
Mariany da Silva CostaBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.
Lúcia Daniela WolfBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.
Fernanda Miyuki KashiwagiBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.
Mariana Abrahão Bueno MoraisBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.
Mikael BodénSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.ORCID 0000-0003-3548-268X
Gerhard SchenkSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.ORCID 0000-0001-8619-0631
Priscila Oliveira de GiuseppeBrazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100, Brazil.ORCID 0000-0003-2094-7325

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biological lignin valorization offers a renewable route to value-added chemicals, yet its industrial implementation is bottlenecked by the inherent instability and low efficiency of

Indexed as

Electron Transport Complex IIIOxygenasesDemethylationLigninMolecular Dynamics SimulationElectron Transport Complex IIILigninOxygenasesRieske iron-sulfur proteinancestral sequence reconstruction (ASR)enzyme engineeringlignin valorizationmolecular dynamics simulationsO-demethylationRieske oxygenaseVanAB system

Identifiers

PMID42234957
PMCPMC13288931

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.