ArticleSTAR protocols2026
Protocol for generating human iPSC-derived cardiomyocytes using VCAM1-based sorting and micropatterned culture.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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5 authors.
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Abstract
The expanding use of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) in disease modeling and regenerative medicine has highlighted persistent challenges, including inter-differentiation variability, immature phenotypes, and heterogeneous cell populations. Here, we present a protocol for generating human iPSC-CMs by combining fluorescence-activated cell sorting (FACS) of VCAM1-positive cells with culture on micropatterned substrates. We describe steps for inducing cardiac differentiation in 3D and enriching iPSC-CMs by FACS. We then detail procedures for maturing iPSC-CMs in 2D micropatterned culture. For complete details on the use and execution of this protocol, please refer to Gomes-Silva et al.
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