Evidence map›Paper›PMID 42234215›Full record

ArticleBiotechnology letters2026

Improved thermostability of Thermobifida fusca mannanase obtained by error-prone PCR mutagenesis.

Yawei Wang, Qiang Xue, Guan Wang, Zhenggang Han, Jiangke Yang, Hairong Xiong

Abstract read
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In one paragraph

Article in Biotechnology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yawei WangCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430048, China.
Qiang XueCollege of Life Science, South-Central Minzu University, Wuhan, 430074, China.
Guan WangCollege of Life Science, South-Central Minzu University, Wuhan, 430074, China.
Zhenggang HanCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430048, China.
Jiangke YangCollege of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430048, China.
Hairong XiongCollege of Life Science, South-Central Minzu University, Wuhan, 430074, China. xionghr@mail.scuec.edu.cn.ORCID http://orcid.org/0000-0002-3829-9613

Funding

the Fund for Academic Innovation Teams of South-Central Minzu University PTZ25027
6 · The paper itself

Abstract

A thermostable β-Mannanase (ManB, PDB code 1BQC, gene code KJ806638) from Thermobifida fusca was expressed in Escherichia coli BL21. The error-prone PCR of ManB gene were adopted to obtain three special mutants with the sites of N15I (M1), D40G/T159I (M2), and H28Q/V57D (M3). It was found that the optimum temperature of wild type ManB was 72.5 °C, whereas of two mutants M1 and M2 were 77.5 °C and mutant M3 was 70 °C. The apparent melting temperatures (T

Indexed as

Actinomycetalesbeta-MannosidaseEnzyme StabilityEscherichia coliHot TemperatureKineticsMutagenesisMutagenesis, Site-DirectedPolymerase Chain ReactionRecombinant ProteinsTemperatureThermobifidabeta-MannosidaseRecombinant ProteinsError-prone PCRMannanaseThermobifida fuscaThermostability

Identifiers

PMID42234215

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.