Evidence map›Paper›PMID 42234076›Full record

ArticleBioresources and bioprocessing2026

CFD-aided design and hydrodynamic characterization of a single-use perfusion bioreactor for high density cell culture.

Yongqiang Liu, Qingfeng Gu, Yu Liu, Guoqian Xu, Yingping Zhuang, Meijin Guo, Chao Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioresources and bioprocessing, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yongqiang LiuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., P.O. box 329#, Shanghai, 200237, People's Republic of China.
Qingfeng GuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., P.O. box 329#, Shanghai, 200237, People's Republic of China.
Yu LiuAlit Biotech (Shanghai) Co., Ltd, Building A8, 66 Yunkai Road, Songjiang District, Shanghai, 201600, People's Republic of China.
Guoqian XuAlit Biotech (Shanghai) Co., Ltd, Building A8, 66 Yunkai Road, Songjiang District, Shanghai, 201600, People's Republic of China.
Yingping ZhuangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., P.O. box 329#, Shanghai, 200237, People's Republic of China.
Meijin GuoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., P.O. box 329#, Shanghai, 200237, People's Republic of China. guo_mj@ecust.edu.cn.ORCID http://orcid.org/0000-0002-3171-4802
Chao LiState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd., P.O. box 329#, Shanghai, 200237, People's Republic of China. leechao1111@163.com.

Funding

Key R&D Program (Science and Technology Demonstration Project) program of Shandong Province, China [No. 2022SFGC0104]National Natural Science Foundation of China [No. 22208099]
6 · The paper itself

Abstract

To address the challenges of high-density animal cell culture, this study developed a high-density culture system comprising a single-use bioreactor (SUB) with a nominal volume of 500 mL and a pulsed tangential flow filtration (ITF) unit. The reactor can be configured with two layers of 35 mm diameter impellers-either double Elephant Ear (EE-EE) or Elephant Ear combined with Ribbon (EE-RB). The flow field characteristics were rigorously characterized through CFD simulations (60-240 rpm; 90-480 mL) validated by experimental data. Engineering analysis revealed the system's robust culture environment: [Formula: see text] values ranging from 2 to 15 h

Indexed as

Computational fluid dynamicsCulture of animal cellsHigh density culturePerfusion cultureShear strain rateSingle-use bioreactor

Identifiers

PMID42234076
PMCPMC13234075

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.