ArticleRSC advances2026
Asymmetric LAMP-gold nanoparticle biosensing for rapid detection of Kenyan tomato leaf curl virus isolates from crude extracts.
Article in RSC advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Authors and funding
4 authors.
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Abstract
Tomato leaf curl virus (ToLCV) is a significant constraint to tomato production, particularly in resource-limited agricultural settings where access to sensitive, decentralized diagnostic tools is limited. While PCR-based detection methods require specialized equipment and exhibit reduced sensitivity when applied to crude field samples, many isothermal methods generate double stranded amplicons that are suboptimal for downstream signal transduction. To address these issues, an asymmetric loop-mediated isothermal amplification (ASYLAMP) strategy was developed to preferentially generate single-stranded amplicons compatible with gold nanoparticle (AuNP) hybridization, enabling rapid visual detection. The optimized ASYLAMP assay achieved shorter time-to-positive results compared to symmetric LAMP while maintaining equivalent specificity, as supported by melt curve analysis and statistical comparison. The assay exhibited a limit of detection of 0.0008 fg µL
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