ArticlePractical laboratory medicine2026
A semi-automatization magnetic solid-phase extraction method of LC-MS/MS for the quantification of homocysteine and its related metabolites in serum.
Article in Practical laboratory medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Clinical mass spectrometry is recognized for its high specificity and sensitivity in quantifying small-molecule biomarkers in serum. However, its broad adoption in clinical settings has been limited by challenges such as low automation and time-consuming workflows. Methods: This study aimed to develop a rapid, sensitive, and automated magnetic solid-phase extraction(MSPE) method using Hydrophilic-Lipophilic Balance (HLB) magnetic bead-based sample preparation coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous quantification of homocysteine(Hcy) and its related nine metabolites. The method was comprehensively validated for specificity, linearity, sensitivity, accuracy, precision, matrix effects, carry-over; and compared it with solid-phase extraction (SPE) methods. Results: Results showed excellent linearity (r Conclusions: In conclusion, the developed semi-automatization method is rapid, highly sensitive, and reproducible, making it suitable for quantitative analysis of these nine homocysteine cycle-related biomarkers in clinical serum samples. It provides a reliable analytical tool for the diagnosis, treatment, and prevention of associated diseases.
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