Evidence map›Paper›PMID 42231953›Full record

ArticleiScience2026

Do G4 ligands induce mitochondrial dysfunction without ROS induction.

Xinru Zhang, Fei Li, Siyi Zeng, Dongyin Lian, Qi Cheng, Lin Li, Xin Gao, Qiantao Wang, Yumei Qin, Junrong Du and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Xinru ZhangKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Fei LiKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Siyi ZengKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Dongyin LianKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Qi ChengKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Lin LiNHC Key Laboratory of Nuclear Technology Medical Transformation, Sichuan Clinical Research Center for Radiation and Therapy, Sichuan Provincial Engineering Research Center of Nuclear Medical Equipment Translation and Application, Department of Nuclear Medicine, Mianyang Central Hospital, Mianyang 621000, China.
Xin GaoNHC Key Laboratory of Nuclear Technology Medical Transformation, Sichuan Clinical Research Center for Radiation and Therapy, Sichuan Provincial Engineering Research Center of Nuclear Medical Equipment Translation and Application, Department of Nuclear Medicine, Mianyang Central Hospital, Mianyang 621000, China.
Qiantao WangKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Yumei QinNHC Key Laboratory of Nuclear Technology Medical Transformation, Sichuan Clinical Research Center for Radiation and Therapy, Sichuan Provincial Engineering Research Center of Nuclear Medical Equipment Translation and Application, Department of Nuclear Medicine, Mianyang Central Hospital, Mianyang 621000, China.
Junrong DuKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Qiang LiDepartment of Thoracic Surgery, Sichuan Cancer Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China.
Zhenlei SongKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Qingrong QiKey Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province, Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Anton GranzhanUniversité Paris Cité, LCBPT, CNRS UMR8601, 75006 Paris, France.
Sophie BombardCNRS-UMR9187, INSERM U1196, Institut Curie, PSL-Research University, 91405 Orsay, France.
Tao Jia 贾涛NHC Key Laboratory of Nuclear Technology Medical Transformation, Sichuan Clinical Research Center for Radiation and Therapy, Sichuan Provincial Engineering Research Center of Nuclear Medical Equipment Translation and Application, Department of Nuclear Medicine, Mianyang Central Hospital, Mianyang 621000, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

G-quadruplex (G4) DNA structures, present in both nuclear and mitochondrial genomes, represent emerging therapeutic targets. Here, we developed and exploited a mitochondrial high-content profiling platform (Mito-HiCP), integrating automated microscopy with quantitative image analysis, to compare the effects of G4 ligands with cisplatin in cancer and normal cells. G4 ligands induce mitochondrial depolarization without reactive oxygen species (ROS) bursts, in contrast to the ROS-associated toxicity of cisplatin. Notably, the G4 ligand 360 A suppresses both mitochondrial and cytosolic ROS levels while retaining tumor selectivity, whereas its non-G4-binding isomer (EDL21) shows no effect, supporting a G4-dependent mechanism. Mechanistically, 360 A reduces mitochondrial DNA copy number, transcription, and electron transport chain protein levels. Using Mito-HiCP, we identify that G4 targeting uncouples mitochondrial membrane potential from ROS production. This reveals a mode of redox regulation that underlies selective anticancer activity and provides a framework to dissect mitochondrial responses and redox regulation across diverse therapeutic perturbations.

Indexed as

Biochemistrycell biologymolecular biology

Identifiers

PMID42231953
PMCPMC13224012

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.