ArticleSynthetic and systems biotechnology2026
Development and clinical validation of a multiplex visual POCT platform based on RT-RAA-CRISPR/Cas12a for porcine diarrheal viruses.
Article in Synthetic and systems biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Development and clinical validation of a multiplex visual POCT platform based on RT-RAA-CRISPR/Cas12a for porcine diarrheal viruses.Synthetic and systems biotechnology · 2026Article
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14 authors.
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Abstract
Porcine epidemic diarrhea virus (PEDV), porcine rotavirus A (PoRVA), and porcine sapelovirus (PSV) are major causative agents of severe diarrhea in swine and frequently occur as co-infections, leading to significant financial losses. To address the urgent demand for rapid, on-site, and multiplex detection, we developed a multiplex reverse transcription recombinase-aided amplification (RT-RAA)-CRISPR/Cas12a assay for visual point-of-care testing (POCT) diagnosis. The assay exhibited high specificity for the three target viruses, with no cross-reactivity against non-target swine pathogens. Under optimized conditions, the system achieved single-copy-level sensitivity, with limits of detection (LOD) of 5.2, 5.9, and 0.57 copies/μL for PEDV, PoRVA, and PSV, respectively, and demonstrated excellent repeatability (coefficient of variation (CV) < 10%). Validation with 20 clinical fecal swabs samples and a portable detection toolbox revealed 95%-100% concordance with standard reverse transcription quantitative polymerase chain reaction (RT-qPCR) and reverse transcription polymerase chain reaction (RT-PCR) methods. The entire "sample-to-answer" workflow was completed within 55 min. This platform provides a powerful and user-friendly tool for POCT of mixed viral infections in swine herds.
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