ArticlePlant cell reports2026
Engineering plant architecture in the ornamental species Eustoma grandiflorum by knockout of strigolactone biosynthesis.
Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
key messageThe targeted mutation of the strigolactone biosynthetic gene, CCD8, through genome-editing CRISPR-Cas9 induces dwarfism and enhanced branching in Eustoma grandiflorum. In ornamental plants, modifying shoot architecture is a major breeding objective for diverse applications; however, achieving this goal using conventional techniques remains challenging. Strigolactones are plant hormones that inhibit shoot branching. We aimed to generate a strigolactone-deficient mutant of Eustoma grandiflorum using CRISPR-Cas9 genome editing. Four guide RNAs targeting CAROTENOID CLEAVAGE DIOXYGENASE 8 (EgCCD8) were designed, and transgenic E. grandiflorum plants were produced. Three independent lines carried deletions or insertions in all EgCCD8 alleles, indicating high editing efficiency. Two lines exhibited increased branching and pronounced dwarfism. In one of these lines, plant height in T
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