ArticleScientific reports2026
Influence of temperature and inoculum composition on standardized biodegradation tests of bioplastics in freshwater under aerobic conditions.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
During the last decades, the production of bioplastics has emerged as a promising alternative to mitigate the environmental impact generated by the accumulation of unmanaged plastic waste on natural ecosystems. Biodegradable bioplastics are expected to be less persistent than conventional oil-based polymers; however, the conditions under which biodegradation tests are conducted may lead to discrepancies between laboratory results and the real environmental conditions. For freshwater ecosystems, previous studies have highlighted important gaps, such as the variability caused by the inoculum. To assess if the use of commercial lyophilized bacteria as inoculum reduces the test variability, the biodegradation of three microplastics (MPs) with distinct polymer origins, poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV), polylactic acid (PLA), and polypropylene (PP), was studied in closed respirometers. Subsequently, following the reduction of the variability, the impact of the incubation temperature was evaluated at 10, 20 and 30 °C. Finally, the potential differences in the biodegradation process caused using different inocula were assessed with three different inoculum sources: the commercial freeze-dried bacteria, an activated sludge and digestate from biowaste. The results showed that freeze-dried bacteria could be used as an alternative inoculum to reduce inter-laboratory differences in the standardized test. Although no statistical differences were observed for PHB under identical incubation conditions with the freeze-dried inoculum, the results showed that the biodegradation of PHBV is highly influenced by temperature with higher values obtained at 35 °C than at 20 °C and 10 °C. Finally, the diversity and relative abundance of the inocula may significantly influence biodegradation outcomes, highlighting the inoculum source as a critical factor contributing to variability in this type of assay.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.