Rumen-derived Pichia membranifaciens modulates the rumen microbiome and metabolome and mitigates methane emissions in dairy cows.

Jichao Li, Xu Liang, Pengyu Liu, Weiyun Zhu, Wei Jin, Shengyong Mao, Fei Xie

Abstract read

In one paragraph

Article in NPJ biofilms and microbiomes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jichao Li *Innovation Centre for Ruminant Nutrition and Cleaner Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Xu Liang *Innovation Centre for Ruminant Nutrition and Cleaner Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Pengyu LiuInnovation Centre for Ruminant Nutrition and Cleaner Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Weiyun ZhuInnovation Centre for Ruminant Nutrition and Cleaner Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Wei JinInnovation Centre for Ruminant Nutrition and Cleaner Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. jinwei@njau.edu.cn.

Shengyong MaoInnovation Centre for Ruminant Nutrition and Cleaner Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. maoshengyong@njau.edu.cn.

Fei XieInnovation Centre for Ruminant Nutrition and Cleaner Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. xiefei_njau@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methane emissions from ruminants represent a significant environmental challenge and dietary energy loss. While yeasts are potential rumen modulators, specific methane-mitigating species remain poorly characterized. Here, we screened 73 rumen-derived strains in vitro, identifying Pichia membranifaciens M12 as the most effective candidate, reducing methane output by 17.1%. Subsequently, a randomized block trial with 36 dairy cows compared a control group with P. membranifaciens M12 supplementation at 2.5 and 5 × 10

Indexed as

MetabolomeMethaneMicrobiotaPichiaRumenAnimal FeedAnimalsBacteriaCattleFemaleLactationMultiomicsMethane

Identifiers

PMID42230654

DOI10.1038/s41522-026-01029-0

PMCPMC13526788

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.