Evidence map›Paper›PMID 42228685›Full record

ArticlePLoS biology2026

miR408-5p and miR408-3p cooperatively reduce cadmium uptake and accumulation in rice.

Fuxi Rong, Yaqi Zhang, Fangrui Ni, Lantian Zhang, Mingxin Yu, Zheyuan Hong, Muhammad Fahad, Yuxin Shen, Chuanjia Liu, Shengke Tian and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Fuxi RongState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Yaqi ZhangState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Fangrui NiState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Lantian ZhangState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Mingxin YuState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Zheyuan HongState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Muhammad FahadState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Yuxin ShenState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Chuanjia LiuState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.
Shengke TianMOE Key Laboratory of Environmental Remediation and Ecological Health, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
Dezhi WuYuelushan Laboratory, College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China.
Liang WuState Key Laboratory of Rice Biology and Breeding, Zhejiang Key Laboratory of Crop Germplasm Innovation and Utilization, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.ORCID https://orcid.org/0000-0003-4953-7258

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In plants, a subset of miRNA precursors can yield multiple mature miRNAs; however, how they simultaneously regulate a single biological process remains poorly understood. Cadmium (Cd) is a non-essential heavy metal toxic to plants, posing serious risks to human health via the food chain. As rice is a major dietary source of Cd, elucidating the molecular mechanisms underlying Cd accumulation is crucial for ensuring food safety. Here, we show that a pair of miRNAs derived from the MIR408 precursor cooperatively represses Cd uptake in roots by targeting distinct genes, consequently reducing Cd accumulation in rice grains. miR408-5p inhibits translation of Heavy metal-associated Isoprenylated Plant Protein 9 (HIPP19), which is specifically expressed in exodermis and endodermis cells and facilitates Cd binding. Meanwhile, miR408-3p cleaves Uclacyanin 7 (UCL7) mRNA, leading to enhance the activity of superoxide dismutases (SODs), and increase production of reactive oxygen species (ROS), particularly hydrogen peroxide (H2O2), which in turn suppresses Cd absorption and accumulation. Furthermore, knockout mutants of HIPP19 and UCL7, as well as MIR408 overexpressing lines, exhibit significantly decreased Cd content in grains, while the accumulation of other essential metals remains comparable to that of wild-type plants. These findings establish a promising strategy for producing "low-Cd rice" without compromising agronomic traits for food safety.

Indexed as

CadmiumMicroRNAsOryzaGene Expression Regulation, PlantHydrogen PeroxidePlant ProteinsPlant RootsReactive Oxygen SpeciesRNA, PlantSuperoxide DismutaseCadmiumHydrogen PeroxideMicroRNAsPlant ProteinsReactive Oxygen SpeciesRNA, PlantSuperoxide Dismutase

Identifiers

PMID42228685
PMCPMC13229367

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.