Evidence map›Paper›PMID 42226673›Full record

ArticleBiotechnology journal2026

A Copper-Inducible Regulatory System for Dynamic Enhancement of Gene Editing Efficiency in Yarrowia lipolytica.

Jiacan Cao, Qing Hong, Jiaxin Sun, Jiaxin Fu, Zhenmin Liu, Baixi Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jiacan CaoState Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd, Shanghai, China.
Qing HongState Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd, Shanghai, China.
Jiaxin SunSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
Jiaxin FuSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
Zhenmin LiuState Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd, Shanghai, China.ORCID https://orcid.org/0000-0003-3777-087X
Baixi ZhangSchool of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.

Funding

State Key Laboratory of Dairy Biotechnology SKLDB2024-002
6 · The paper itself

Abstract

To circumvent the potential irreversible risks to cells posed by permanent genetic modifications, this study developed a copper-inducible system for dynamically regulating gene editing efficiency of Yarrowia lipolytica. By combining promoters pMT2 or enhanced pUAS16MT2 with genes scRAD52, scRAD59, and ylRAD52, homologous recombination efficiency was raised from 21.4% to 56.4%, while maintaining a transformation efficiency of 75 ± 5 CFU/OD

Indexed as

CopperGene EditingYarrowiaAcyl-CoA OxidaseGene Knockout TechniquesHomologous RecombinationPromoter Regions, GeneticAcyl-CoA OxidaseCopperconjugated linolenic aciddynamic regulationhomologous recombinationYarrowia lipolytica

Identifiers

PMID42226673
PMCPMC13383611

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.