Evidence map›Paper›PMID 42226053›Full record

ArticleBMC veterinary research2026

An IgM monoclonal antibody targeting diguanylate cyclase DgcE potentiates gentamicin activity against avian pathogenic Escherichia coli through modulation of c-di-GMP signaling.

Li Fangfang, Nie Lianhua, Wang Haiyang, Wang Zhihao, Lian Liyan, Hu Jiangang, Jia Yuanzheng, Bao Yinli, Han Xiangan, Wang Haidong

Abstract read
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In one paragraph

Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Li FangfangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, P. R. China.
Nie LianhuaCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, P. R. China.
Wang HaiyangCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, P. R. China.
Wang ZhihaoShanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, China.
Lian LiyanShanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, China.
Hu JiangangShanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, China.
Jia YuanzhengShanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, China.
Bao YinliKey Laboratory of Fujian Universities Preventive Veterinary Medicine and Biotechnology, Longyan University, Longyan, 364012, China.
Han XianganShanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, China. hanxgan@163.com.
Wang HaidongCollege of Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, P. R. China. wanghaidong@sxau.edu.cn.

Funding

Fujian Provincial Natural Science Foundation 2025J011021Natural Science Foundation of Shanghai Municipality 22ZR1475800Open Fund of the Fujian Provincial Key Laboratory of Preventive Veterinary Medicine and Biotechnology (Longyan University) 2025KF03the National Natural Science Foundation of China U22A20518the Scientific and Technological Innovation Enhancement Project of Shanxi Agricultural University CXGC2025010the Start-up Fund for Doctoral Research at Shanxi Agricultural University 2026BQ07
6 · The paper itself

Abstract

backgroundThe combination of monoclonal antibodies with aminoglycosides represents a promising strategy to counter the increasing prevalence of aminoglycoside resistance in avian pathogenic E. coli (APEC). Although the diguanylate cyclase DgcE (UniProt: P38097) is known to regulate virulence through c-di-GMP signaling, its potential as a therapeutic target has not been investigated.

resultsIn this study, five female BALB/c mice were immunized to generate a monoclonal antibody. We developed an IgM monoclonal antibody (E11G12) targeting a defined region of DgcE and evaluated its ability to potentiate gentamicin activity. Structural prediction and docking analyses suggested specific binding interactions within the targeted DgcE region. In vitro checkerboard assays demonstrated synergistic activity between E11G12 and gentamicin (FICI ≤ 0.5), and time-kill assays showed enhanced bactericidal activity compared to monotherapy. Treatment was associated with reduced intracellular c-di-GMP levels and increased gentamicin accumulation.

conclusionThese findings suggest that the E11G12-aminoglycoside combination exhibits synergistic anti-APEC activity, potentially through blocking virulence via DgcE inhibition and enhancing antibiotic efficacy in association with c-di-GMP suppression. This approach offers a novel therapeutic strategy against drug-resistant APEC infections.

Indexed as

Anti-Bacterial AgentsAntibodies, MonoclonalCyclic GMPEscherichia coliEscherichia coli InfectionsEscherichia coli ProteinsGentamicinsImmunoglobulin MPhosphorus-Oxygen LyasesAnimalsDrug SynergismFemaleMiceMice, Inbred BALB CSignal TransductionAnti-Bacterial AgentsAntibodies, Monoclonalbis(3',5')-cyclic diguanylic acidCyclic GMPdiguanylate cyclaseEscherichia coli ProteinsGentamicinsImmunoglobulin MPhosphorus-Oxygen LyasesAminoglycoside resistanceAvian Pathogenic Escherichia coli (APEC)Cyclic di-GMPDiguanylate cyclase DgcEMonoclonal antibody

Identifiers

PMID42226053
PMCPMC13440229

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.