Evidence map›Paper›PMID 42225069›Full record

ArticleCell reports methods2026

Rapid neuronal labeling and functional imaging in the developing mouse brain with AAV-PHP.eB.

Weiyi Ye, Mao Deng, Sijing Zhao, Xiaodong Chen, Simin Su, Xiaoqin Lian, Bowen Ren, Chuchu Qi, Jialong Li, Mei Fu and 8 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Weiyi YeZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528403, China.
Mao DengZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528403, China.
Sijing ZhaoSchool of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210032, China.
Xiaodong ChenZunyi Medical University, Zunyi 563000, China.
Simin SuShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 101408, China.
Xiaoqin LianGuizhou Medical University, Guiyang 561113, China.
Bowen RenDivision of Medicine, University College London, Royal Free Campus, London WC1E 6BT, UK.
Chuchu QiDepartment of Neurobiology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Jialong LiDepartment of Neurobiology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China.
Mei FuZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528403, China.
Tongtong GaoZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528403, China; Faculty of Medicine, Macau University of Science and Technology, Macao 999078, China.
Zhihan BaiShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 101408, China.
Minghan LiDepartment of Computational Biology, School of Life Sciences, Fudan University, Shanghai 200433, China.
Dihan LuDepartment of Anesthesiology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China.
Na ZhouFaculty of Medicine, Macau University of Science and Technology, Macao 999078, China.
Shiqian ShenDepartment of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston MA 02171, USA.
Wenting WangDepartment of Neurobiology, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China. Electronic address: wwt0657@fmmu.edu.cn.
Qian ChenZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528403, China; Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 101408, China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210032, China; Zunyi Medical University, Zunyi 563000, China; Guizhou Medical University, Guiyang 561113, China. Electronic address: chenqian@zidd.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neuronal labeling in the developing brain is essential for studying the formation and maturation of neural circuits. Among labeling techniques, adeno-associated virus (AAV)-based gene delivery offers high efficiency and versatility. However, whether AAV can effectively label neurons in the developing brain has not been fully determined. Here, we demonstrate that administration of AAV-PHP.eB in neonatal mice results in neuronal transduction as early as 3 days after injection. By titrating the AAVs, we achieve sparse neuronal labeling and facilitate high-resolution reconstruction of dendritic morphology during the critical developmental period. Then, we extend this method to functional imaging by delivering GCaMP, allowing longitudinal in vivo two-photon calcium imaging in the immature brain. Our findings show that AAV-PHP.eB-mediated early postnatal delivery offers a rapid, efficient, and minimally invasive approach for neuronal labeling and functional imaging in the developing brain, providing opportunities to investigate neural circuit development at both structural and functional levels.

Indexed as

BrainDependovirusNeuronsStaining and LabelingAnimalsCalciumGenetic VectorsMiceTransduction, GeneticCalciumAAV-PHP.eBcalcium imagingCP: imagingCP: neurosciencedeveloping brainGCaMPsingle-neuron labeling

Identifiers

PMID42225069
PMCPMC13390054

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.