ArticleSTAR protocols2026
Protocol for untargeted lipidomics of human serum using LC-TIMS-PASEF.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
2 authors.
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Abstract
Here, we present a protocol for untargeted lipidomics of human serum. We describe steps for project design, sample preparation, and reversed-phase liquid chromatography coupled with trapped ion mobility spectrometry and parallel accumulation serial fragmentation (LC-TIMS-PASEF). We then detail procedures for quality control and data processing. The protocol enables comprehensive lipid profiling from 5 μL of serum, yielding annotations across glycerophospholipids, glycerolipids, sphingolipids, sterols, and fatty acyls. The resulting datasets support statistical analysis and integration with complementary omics approaches.
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Registered trials
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