Evidence map›Paper›PMID 42222665›Full record

ArticleSoftwareX2025

AlliGator: Open source fluorescence lifetime imaging analysis in G.

Xavier Michalet

Abstract read
In one paragraph

Article in SoftwareX, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. AlliGator: Open Source Fluorescence Lifetime Imaging Analysis in G.bioRxiv : the preprint server for biology · 2025
    Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Xavier MichaletDepartment of Chemistry & Biochemistry, University of California at Los Angeles, 607 Charles E. Young Drive E, Los Angeles, CA 90095, United States of America.ORCID 0000-0001-6602-7693

Funding

In vivo Macroscopic Fluorescence Lifetime Molecular Optical ImagingR01CA250636 · NCI · RENSSELAER POLYTECHNIC INSTITUTE · PI BARROSO, MARGARIDA, INTES, XAVIER · 2020 to 2023
$2.9M
Single-photon counting APD arrays for high-through-put single-molecule assaysR01GM095904 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GHIONI, MASSIMO ANTONIO, MICHALET, XAVIER · 2012 to 2016
$2.0M
NCI NIH HHS R01 CA250636NIGMS NIH HHS R01 GM095904
6 · The paper itself

Abstract

Fluorescence Lifetime Imaging (FLI) is a technique recording the temporal decay of fluorescence emission at every pixel of an image. Analyzing the information embedded in FLI dataset requires either fitting the decay to a predefined model using nonlinear least-square fit or maximum likelihood estimation, or projecting the decay on an orthogonal basis of periodic functions as in phasor analysis. AlliGator is a Windows open source software (BSD license) implementing these approaches in a user-friendly graphical user interface (GUI) and offering numerous unique features such as Förster Resonant Energy Transfer (FRET) stoichiometry analysis, or the creation of maps of lifetime-derived quantities such as membrane potential. It leverages the unique ability of the LabVIEW graphical programming language (G) to design feature-rich GUI, and supports user-developed plugins written in python to extend its native capabilities.

Indexed as

Fluorescence lifetime imagingLabVIEWNLSFPhasor analysisPython

Identifiers

PMID42222665
PMCPMC13220918

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.