A Dual-Emissive Organoplatinum(II) Probe Enables Ratiometric NIR and Phosphorescence Lifetime Imaging of Cell Membrane Integrity.

Cheng Ouyang, Zhuoli Chen, Yu Chen, Hui Chao

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Article in Chemical & biomedical imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Cheng OuyangMOE Key Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Anti-Infective Drug Discovery and Development, Guangdong Basic Research Center of Excellence for Functional Molecular Engineering, School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, P. R. China.

Zhuoli ChenMOE Key Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Anti-Infective Drug Discovery and Development, Guangdong Basic Research Center of Excellence for Functional Molecular Engineering, School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, P. R. China.

Yu ChenMOE Key Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Anti-Infective Drug Discovery and Development, Guangdong Basic Research Center of Excellence for Functional Molecular Engineering, School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, P. R. China.ORCID https://orcid.org/0000-0001-5219-9195

Hui ChaoMOE Key Laboratory of Bioinorganic and Synthetic Chemistry, State Key Laboratory of Anti-Infective Drug Discovery and Development, Guangdong Basic Research Center of Excellence for Functional Molecular Engineering, School of Chemistry, Sun Yat-Sen University, Guangzhou 510006, P. R. China.ORCID https://orcid.org/0000-0003-4153-5303

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescent microscopy imaging continues to face major challenges due to biological scattering and autofluorescence. In this work, tridentate platinum-(II) complexes self-assemble into nanoaggregates via Pt-Pt interactions, enabling molecular-level control over the cellular uptake discrimination between membrane-intact and disrupted cells. This self-assembly switches the emission character from intraligand charge transfer (ILCT) to a lower-energy metal-metal-to-ligand charge transfer (MMLCT), yielding a ratiometric blue-to-NIR photoluminescence response. The emission transition is accompanied by a prolongation of the luminescence lifetime into the microsecond regime, which facilitates concentration-independent lifetime imaging. Furthermore, rational ligand engineering enhances the two-photon absorption cross-sections of the complexes, supporting their use in 3D imaging. The combination of assembly-driven two-photon excited NIR ratiometric emission and lifetime differentiation offers an approach for real-time monitoring of cell membrane integrity.

Indexed as

bioimagingNIR Imagingphosphorescence lifetime imagingplatinumratiometric dual emissiontwo-photon excitation

Identifiers

PMID42222564

DOI10.1021/cbmi.5c00206

PMCPMC13217326

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