Evidence map›Paper›PMID 42222498›Full record

ArticlePeerJ2026

Development of a multiplex recombinase polymerase amplification combined with a DNA strip for the rapid detection of

Pattharasak Thaweesub, Aroonlug Lulitanond, Aroonwadee Chanawong, Setchanaphong Thitiwatrueangrut, Pheeraphon Khlaichanthong, Patcharaporn Tippayawat, Molin Wongwattanakul, Orapan Sripichai, Arpasiri Srisrattakarn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Pattharasak ThaweesubCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Aroonlug LulitanondCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Aroonwadee ChanawongCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Setchanaphong ThitiwatrueangrutCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Pheeraphon KhlaichanthongCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
Patcharaporn TippayawatCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.ORCID 0000-0002-4452-1284
Molin WongwattanakulCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.ORCID 0000-0002-8494-4142
Orapan SripichaiNational Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.ORCID 0000-0002-0798-8934
Arpasiri SrisrattakarnCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Vancomycin-resistant enterococci (VRE), particularly Methods: A total of 106 samples, including 46 Results: Compared with the PCR-based method, the M-RPA-C-PAS method demonstrated sensitivities of 95.7%, 94.9%, and 100.0% and specificities of 100.0%, 92.5%, and 100.0% for Conclusion: The M-RPA-C-PAS method is an alternative rapid approach with high sensitivity and specificity, demonstrating comparable performance to conventional PCR. Therefore, the M-RPA-C-PAS method represents a promising point-of-care diagnostic tool for the rapid detection of VRE, supporting timely clinical management and infection control strategies.

Indexed as

Enterococcus faecalisEnterococcus faeciumMultiplex Polymerase Chain ReactionVancomycin ResistanceBacterial ProteinsCarbon-Oxygen LigasesDNA, BacterialGram-Positive Bacterial InfectionsHumansNucleic Acid Amplification TechniquesRapid Diagnostic TestsRecombinasesSensitivity and SpecificityBacterial ProteinsCarbon-Oxygen LigasesDNA, BacterialRecombinasesVanA ligase, BacteriaChromatographic printed-array stripEnterococcus faecalisEnterococcus faeciumRecombinase polymerase amplificationVancomycin-resistant enterococci

Identifiers

PMID42222498
PMCPMC13221993

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.