ArticleFrontiers in microbiology2026
Development and validation of a Vero cell DNA standard for residual DNA measurement suitable for quantitative PCR method.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: The fluorescence quantitative PCR method for the detection of Vero cell residual DNA in human rabies vaccine urgently needs a national standard for Vero cell DNA quantification. Methods: Briefly, the genomic DNA of Vero cells was extracted, diluted to 100 μg/ml, and divided into 0.1-ml aliquots in sterile screw cap tubes. After DNA preparation, concentration calibration, applicability, and stability verification were carried out by multiple laboratories. Results: The concentration of the national standard for Vero cell DNA quantification (limited qPCR method) determined from this collaborative study was 107 ± 6 μg/mL, and the DNA purity (OD Conclusion: This study successfully developed a national standard for Vero cell DNA quantification using the quantitative fluorescence PCR method, which has significant application potential for standardizing Vero cell DNA residue detection in human rabies vaccines.
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