ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2026
Evaluation of the FMDV 2.1 dry RT-rtPCR prototype assay for detection of foot-and-mouth disease virus in lymph nodes.
Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
We evaluated the performance of the FMDV 2.1 dry RT-rtPCR prototype assay (Tetracore) to detect foot-and-mouth disease virus (FMDV) in lymph nodes. Various masses (20-100 mg) of FMDV-negative lymph node tissue were homogenized, diluted, and spiked with FMDV RNA to determine the optimal amount for detection. Optimal amplification was obtained using 20 mg of tissue. Fifteen lymph nodes collected from animals challenged with FMDV that had clinical signs were subjected to manual and automated homogenization and tested using the FMDV 2.1 dry RT-rtPCR prototype assay on a T-COR 8 thermocycler (Tetracore). Additionally, automated homogenates were tested using the World Organisation for Animal Health (WOAH)-recommended RT-rtPCR assay on a CFX real-time thermocycler (Bio-Rad). Strong Pearson correlation was observed between homogenization methods using the FMDV 2.1 dry RT-rtPCR assay (
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