ArticleFunctional & integrative genomics2026
Transgene-free plant genome editing via viral delivery of miniature CRISPR-Cas12f.
Article in Functional & integrative genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Efficient delivery of CRISPR reagents into plant cells remains a major bottleneck, particularly for transgene-free genome editing. RNA viral vectors such as Tabacco Rattle Virus (TRV) provide an attractive platform for transient expression but are limited by their small cargo capacity. Recently discovered miniature nucleases like Cas12f, due to their compact size and high activity, offer a promising alternative for viral delivery systems. In this study, a plant codon-optimized Acidibacillus sulfuoxidans Cas12f (AsCas12f), fused with nuclear localization signals, was cloned into the TRV genome to develop a viral vector-based delivery platform. Two sgRNAs targeting the Nicotiana benthamiana PHYTOENE DESATURASE (NbPDS) gene were delivered through Agrobacterium-mediated infiltration. To enhance mobility and transcript abundance, mobile RNA elements such as modified Flowering Locus T (mFT), truncated FLOWERING LOCUS T (tFT), and transfer RNA of methionine (tRNA
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