Single-cell transcriptome analysis reveals SOX9 mutation-driven tumor stemness and microenvironment remodeling through the COL1A1-CD44 axis in colorectal cancer.

Yuwei Pan, Xuelian Xiang, Junfeng Li, Yinggang Ge, Xiaoyan Liang

Abstract read

In one paragraph

Article in BMC cancer, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Pericyte-derived cancer-associated fibroblasts correlate with poor survival and are enriched after chemoradiotherapy in glioblastoma.PloS one · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yuwei PanDepartment of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Xuelian XiangDepartment of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Junfeng LiDepartment of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Yinggang GeDepartment of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. gyg02315@sina.com.

Xiaoyan LiangDepartment of Gastroenterology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. lxyan@hospital.cqmu.edu.cn.

Funding

Chongqing Young and Middle-aged Medical High-level Talent Program No. 2023GDRC009Natural Science Foundation of Chongqing No. CSTB2022NSCQMSX0803the National Nature Science Foundation of China No. 82273125

6 · The paper itself

Abstract

Colorectal cancer (CRC) exhibits substantial interpatient heterogeneity and is driven by intricate crosstalk between tumor-intrinsic mutations and the tumor microenvironment (TME), yet the mechanisms by which specific genetic alterations remodel the TME and regulate tumor stemness remain elusive. The SRY-box transcription factor 9 (SOX9) is a key regulator of intestinal homeostasis, with mutations implicated in CRC progression, but its single-cell level effects on TME dynamics have not been fully elucidated. To dissect the heterogeneity and cell-cell interactions underlying SOX9 mutation-driven CRC progression, we integrated single-cell RNA sequencing (scRNA-seq) data of 23 CRC tumor samples with bulk sequencing and mutation data from The Cancer Genome Atlas (TCGA) using the Scissor algorithm. We identified a malignant epithelial subpopulation (Scissor

Indexed as

Collagen Type IColorectal NeoplasmsHyaluronan ReceptorsNeoplastic Stem CellsSOX9 Transcription FactorTumor MicroenvironmentAnimalsCell Line, TumorCollagen Type I, alpha 1 ChainGene Expression ProfilingGene Expression Regulation, NeoplasticHumansMiceMutationSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisCD44 protein, humanCollagen Type ICollagen Type I, alpha 1 ChainHyaluronan ReceptorsSOX9 protein, humanSOX9 Transcription FactorCOL1A1–CD44 axisColorectal cancerSingle-cell RNA sequencingSOX9 mutationTumor stemness

Identifiers

PMID42218401

DOI10.1186/s12885-026-16248-z

PMCPMC13430886

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.