Evidence map›Paper›PMID 42218270›Full record

ArticleScientific reports2026

Effects of fluoroquinolone antibiotics on extracellular matrix-related phenotypes in tendon cells.

Akshay Anand, Keiko Sakai, David Dickens, Sadatoshi Tsuzuki, Sachiko Minamiguchi, Naoya Asai, Ahmed Kazaili, Riaz Akhtar, Munir Pirmohamed, Takao Sakai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Akshay AnandCentre for Drug Safety Science, Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 3GE, UK.
Keiko SakaiCentre for Drug Safety Science, Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 3GE, UK.
David DickensCentre for Drug Safety Science, Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 3GE, UK.
Sadatoshi TsuzukiDepartment of Diagnostic Pathology, Fujita Health University School of Medicine, 1-98, Dengakugakubo, Kusukakecho, Toyoake, Aichi, 470-1192, Japan.
Sachiko MinamiguchiDepartment of Diagnostic Pathology, Fujita Health University School of Medicine, 1-98, Dengakugakubo, Kusukakecho, Toyoake, Aichi, 470-1192, Japan.
Naoya AsaiDepartment of Pathology, Fujita Health University School of Medicine, 1-98, Dengakugakubo, Kusukakecho, Toyoake, Aichi, 470-1192, Japan.
Ahmed KazailiDepartment of Materials, Design and Manufacturing Engineering, School of Engineering, University of Liverpool, Liverpool, L69 3GH, UK.
Riaz AkhtarDepartment of Materials, Design and Manufacturing Engineering, School of Engineering, University of Liverpool, Liverpool, L69 3GH, UK.
Munir PirmohamedCentre for Drug Safety Science, Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 3GE, UK.
Takao SakaiCentre for Drug Safety Science, Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool, L69 3GE, UK. sirotakake@gmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluoroquinolones (FQ) are amongst the most widely prescribed antibiotics used to treat a wide range of infections because of their excellent gastrointestinal absorption, superior tissue penetration and broad-spectrum activity. However, FQs can cause rare, though serious, collagen-associated adverse reactions, including tendinopathy, and there is still no clear framework for understanding the phenotypes relating to how tenocyte-produced extracellular matrix (ECM) and related molecules change in response to FQs. Here, we show carrier-mediated intracellular uptake of the FQ ciprofloxacin by mouse tendon cells in vitro. FQ antibiotics downregulated not only the production of the main component of tendon ECM, type I collagen, but also its assembly capacity and hydroxyproline content, which was accompanied by reduced fibronectin production. Treatment of tendon cells with ciprofloxacin downregulated the enzymatic activity of lysyl oxidase and the stiffness and diameter of collagen fibrils in vitro. Furthermore, ciprofloxacin-treated tendon cells exhibited decreased expression of active β1-integrin, the key ECM receptor. Thus, the reduction in fibronectin production and active β1-integrin expression support the notion that anoikis could be the pathological basis for FQ-induced tendinopathy together with adverse phenotypes that involve type I collagen.

Indexed as

Anti-Bacterial AgentsCiprofloxacinExtracellular MatrixFluoroquinolonesTendonsTenocytesAnimalsCells, CulturedCollagen Type IFibronectinsIntegrin beta1MicePhenotypeProtein-Lysine 6-OxidaseTendinopathyAnti-Bacterial AgentsCiprofloxacinCollagen Type IFibronectinsFluoroquinolonesIntegrin beta1Protein-Lysine 6-OxidaseAdult tendon cellsExtracellular matrixFibronectinFluoroquinolone antibioticsType I collagenβ1-integrin

Identifiers

PMID42218270
PMCPMC13454159

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.