ArticleScientific reports2026
Analysis of purines and pyrimidines across biospecimens: influence of storage temperature and duration.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Evidence-based standardization of infectious disease biobanking: aligning specimen preservation and clinical metadata withFrontiers in public health · 2026Article
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Authors and funding
10 authors.
Funding
Abstract
Purines and pyrimidines are key cellular components for the development, proliferation, and repair of cells and tissues, whose circulating levels are associated with various diseases. Biospecimen processing delays can significantly induce hypoxanthine and inosine levels, among other changes, in blood. We developed a liquid chromatography tandem mass-spectrometry method to quantify metabolites of the purine and pyrimidine pathways and investigated the impact of delayed sample processing on single donor plasma and dried blood spots (DBS). Plasma hypoxanthine increased above the normal reference range to an average 20-fold increase across individual donors when whole blood was kept refrigerated 64 h prior to processing. The same trend was observed in dried blood spots (DBS) prepared from refrigerated whole blood. Plasma inosine also increased above the reference range after a 48-hour delay in processing refrigerated whole blood. In contrast, hypoxanthine remained stable in DBS for up to 48 h, regardless of the card storage temperature. Therefore, DBS is a feasible alternative specimen type to measure hypoxanthine if frozen shipping, delayed centrifugation, or storage interval is a concern. Understanding the impact of specimen handling conditions is essential for successful biomarker discovery, especially when new biomarkers are to be introduced into the clinical laboratory.
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