Evidence map›Paper›PMID 42216500›Full record

ArticleMolecular plant pathology2026

The Role of Two Glycoalkaloid Metabolism Genes in α-Tomatine Biosynthesis and Basal Defence in Tomato.

Yaohua You, Aishwarya Balaji, Andrea Lorena Herrera Valderrama, Marie-Emma Denarié, H M Suraj, Miguel Ramirez Gaona, Katharina Hanika, Francel Verstappen, Iris F Kappers, Jan A L van Kan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular plant pathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yaohua YouLaboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.
Aishwarya BalajiLaboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.
Andrea Lorena Herrera ValderramaLaboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.
Marie-Emma DenariéLaboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.ORCID 0009-0004-5566-4071
H M SurajLaboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.
Miguel Ramirez GaonaLaboratory of Plant Breeding, Wageningen University, Wageningen, the Netherlands.
Katharina HanikaLaboratory of Plant Breeding, Wageningen University, Wageningen, the Netherlands.
Francel VerstappenLaboratory of Plant Physiology, Wageningen University, Wageningen, the Netherlands.
Iris F KappersLaboratory of Plant Physiology, Wageningen University, Wageningen, the Netherlands.
Jan A L van KanLaboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.

Funding

China Scholarship Council
6 · The paper itself

Abstract

Steroidal glycoalkaloids and saponins are plant cholesterol-based steroid metabolites with antimicrobial activities and potential pharmacological value. The saponin uttroside B from black nightshade (Solanum nigrum) plays an important role in defence against herbivorous insects and exhibits anti-hepatocellular carcinoma activity. The tomato (Solanum lycopersicum) glycoalkaloid α-tomatine has been studied because of its antinutritional effects; however, its role in protecting plants from fungal pathogens remains understudied. The biosynthetic pathway of α-tomatine involves multiple clustered genes designated as glycoalkaloid metabolism (GAME) genes. In this study, we generated single knockout mutants of SlGAME4 and SlGAME2 by CRISPR/Cas9-based genome editing. The SlGAME4 mutants did not accumulate glycoalkaloids but instead redirected resources towards steroidal saponin (uttroside B) synthesis. SlGAME2 mutants contained unaltered α-tomatine contents, indicating that the SlGAME2 gene, previously reported to catalyse the transfer of xylose to β

Indexed as

AlkaloidsGenes, PlantSolanum lycopersicumTomatineBotrytisGene Expression Regulation, PlantMutationPlant DiseasesPlant ProteinsAlkaloidsalpha-tomatinePlant ProteinsTomatine

Identifiers

PMID42216500
PMCPMC13239889

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.