Complete genome sequence of Bacillus pseudomycoides CHAES I 2_2, a PHA-producing bacterium isolated from benthal deposits of the chornobyl NPP water cooling pond.

objectivesBacillus pseudomycoides, a member of the Bacillus cereus group, is widely distributed in soil and plant-associated environments, where it contributes to ecological interactions through the production of diverse bioactive compounds. Strains of this species are recognized for their capacity to synthesize compounds that enhance stress tolerance and promote overall environmental persistence. In this study, we evaluated the metabolic potential of B. pseudomycoides CHAES I_2_2, isolated from Chornobyl NPP water cooling pond sediments, using whole-genome analysis to characterize radiation resistance determinants and biotechnologically relevant secondary metabolite biosynthetic gene clusters. DATA DESCRIPTION: We isolated Bacillus pseudomycoides CHAES I_2_2 from benthic sediments of the Chornobyl NPP cooling pond and generated its draft genome sequence. The assembled genome comprises 5,154,520 bp with 35.5% GC content. Genome annotation identified 60 tRNAs, 5 rRNAs, and 4,955 protein-coding sequences. Genome analysis confirmed the presence of a complete polyhydroxyalkanoate biosynthesis gene cluster. The strain possessed genes involved in radiation stress adaptation, including DNA repair, oxidative stress response, sporulation, as well as a paeninodin-like lassopeptide biosynthetic gene cluster and a bacillibactin-type NRPS siderophore cluster. These genomic findings underscore the stress resilience and biotechnological potential of the studied strain.