Evidence map›Paper›PMID 42212855›Full record

ArticleAnalytical chemistry2026

Isotope Decluttering Reduces Spectral Complexity while Maintaining Protein Structure.

Sneha S Hakke, Willem E M Noteborn, Birol Cabukusta, Ye Gao, Kèvin Knoops, Carmen López-Iglesias, David P A Kilgour, David J Clarke, Anjusha Mathew, Ron M A Heeren

Abstract read
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In one paragraph

Article in Analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sneha S HakkeMaastricht Multi-Modal Molecular Imaging Institute (M4i), Maastricht University, 6229 ER Maastricht, The Netherlands.ORCID 0009-0002-3750-4125
Willem E M NotebornNetherlands Centre for Electron Nanoscopy (NeCEN), Leiden University, 2300 RS Leiden, The Netherlands.
Birol CabukustaMaastricht Multi-Modal Molecular Imaging Institute (M4i), Maastricht University, 6229 ER Maastricht, The Netherlands.
Ye GaoMaastricht Multi-Modal Molecular Imaging Institute (M4i), Maastricht University, 6229 ER Maastricht, The Netherlands.
Kèvin KnoopsMicroscopy CORE Lab, Maastricht University, 6229 ER Maastricht, The Netherlands.ORCID 0000-0002-7539-1160
Carmen López-IglesiasMicroscopy CORE Lab, Maastricht University, 6229 ER Maastricht, The Netherlands.
David P A KilgourVibrat-Ion Ltd, Aberystwyth SY23 3AF, U.K.
David J ClarkeEaStCHEM School of Chemistry, University of Edinburgh, Edinburgh, Scotland EH9 3FJ, U.K.ORCID 0000-0002-3741-2952
Anjusha MathewMaastricht Multi-Modal Molecular Imaging Institute (M4i), Maastricht University, 6229 ER Maastricht, The Netherlands.
Ron M A HeerenMaastricht Multi-Modal Molecular Imaging Institute (M4i), Maastricht University, 6229 ER Maastricht, The Netherlands.ORCID 0000-0002-6533-7179

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Accurate mass determination is one of the fundamental objectives in mass spectrometry (MS) as it enables confident molecular identification and detection of subtle mass differences. Precise mass determination typically relies on the measurement of the monoisotopic peak. The increasing number of heavier isotopes, as the molecular mass increases, leads to spectral complexity, broadening of the isotopic distribution, and dispersal of signal intensity, ultimately reducing the signal-to-noise ratio (SNR). These effects are enhanced while analyzing larger proteins and protein complexes. In this study, we expressed and purified two

Indexed as

Bacterial ProteinsCytochrome b GroupFerritinsCarbon IsotopesMass SpectrometryMycobacterium tuberculosisNitrogen IsotopesProtein ConformationSignal-To-Noise RatioBacterial ProteinsbacterioferritinCarbon IsotopesCytochrome b GroupFerritinsNitrogen Isotopes

Identifiers

PMID42212855
PMCPMC13262625

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.