Evidence map›Paper›PMID 42212637›Full record

ArticlePlant, cell & environment2026

TaWRKY33 Positively Regulates TaERF1-A, Thereby Activating TaP5CS

Yang Yu, Yaning Bu, Tianqi Song, Xiaoxing Wang, Dingguo Zhang, Shuangxing Zhang, Hongwei Zhou, Weijun Zhang, Dongsheng Chen, Jishan Xiang and 1 more

Abstract read
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In one paragraph

Article in Plant, cell & environment, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yang YuCollege of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.ORCID https://orcid.org/0000-0003-2273-389X
Yaning BuCollege of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.
Tianqi SongCollege of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.ORCID https://orcid.org/0000-0001-6402-1212
Xiaoxing WangXinjiang Key Laboratory of Lavender Conservation and Utilization, Yili Normal University, Xinjiang, Yili, China.
Dingguo ZhangXinjiang Key Laboratory of Lavender Conservation and Utilization, Yili Normal University, Xinjiang, Yili, China.
Shuangxing ZhangCollege of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.
Hongwei ZhouCollege of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.ORCID https://orcid.org/0000-0001-6775-1165
Weijun ZhangCrop Research Institute, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia, China.
Dongsheng ChenCrop Research Institute, Ningxia Academy of Agriculture and Forestry Science, Yinchuan, Ningxia, China.
Jishan XiangXinjiang Key Laboratory of Lavender Conservation and Utilization, Yili Normal University, Xinjiang, Yili, China.
Xiaoke ZhangCollege of Agronomy, Northwest A&F University, Yangling, Shaanxi, China.

Funding

Key Research and Development Project of Ningxia Hui Autonomous Region 2023BCF01011National Key Research and Development Project of China 2022YFD1200903Tianchi Talent Introduction Program of Xinjiang Autonomous Region 2024TCYCCXLJ01Xinjiang Tianchi Talents Introduction ProgramYili Normal University 2023RCYJ07Young Science and Technology Talent Support Project TAASTJ2026-004
6 · The paper itself

Abstract

Drought stress severely limits global wheat productivity. Herein, the function of TaERF1-A, encoded by a previously undescribed wheat ERF gene, in enhancing drought stress tolerance was analyzed. TaERF1-A exhibited nuclear localisation and transcriptional activation activity in planta, although no transactivation was detected in yeast. Notably, TaERF1-A was upregulated under drought and salt stress. TaERF1-A overexpression (OE) in Arabidopsis enhanced drought stress resistance, resulting in increased transcription levels of stress- and antioxidant-enzyme-related genes. BSMV-TaERF1-A-silenced wheat plants exhibited exacerbated wilting under drought, accompanied by elevated malondialdehyde levels, reduced proline accumulation, and decreased antioxidant enzyme activity levels. In contrast, TaERF1-A OE wheat lines exhibited enhanced drought tolerance, with opposite physiological trends. Notably, RNAi induced a weakened response in wheat compared to wild-type wheat. Yeast one-hybrid, electrophoretic mobility shift, dual-luciferase, and qRT-PCR assays demonstrated that TaWRKY33 directly binds the TaERF1-A promoter, thereby activating its transcription. TaWRKY33 overexpression also enhanced drought tolerance in wheat. TaERF1-A subsequently regulated TaP5CS2 expression, which stimulated proline biosynthesis, thereby contributing to improved drought resistance. This study elucidates the mechanism by which the TaWRKY33-TaERF1-A-TaP5CS2 module positively regulates drought tolerance and highlights the candidate genes that can be explored for developing elite drought-resistant wheat varieties.

Indexed as

Plant ProteinsProlineTranscription FactorsTriticumArabidopsisArabidopsis ProteinsDrought ResistanceDroughtsGene Expression Regulation, PlantGlutamate-5-Semialdehyde DehydrogenaseMultienzyme ComplexesPhosphotransferases (Alcohol Group Acceptor)Plants, Genetically ModifiedPromoter Regions, GeneticStress, PhysiologicalTranscriptional ActivationArabidopsis Proteinsdelta(1)-pyrroline-5-carboxylate synthetase, ArabidopsisGlutamate-5-Semialdehyde DehydrogenaseMultienzyme ComplexesPhosphotransferases (Alcohol Group Acceptor)Plant ProteinsProlineTranscription Factorsdrought toleranceERFTaERF1‐ATaP5CS2TaWRKY33wheat

Identifiers

PMID42212637
PMCPMC13436439

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.