ArticleHuman mutation2026
Single-Cell Transcriptomic Analysis Suggests That JUN May Regulate BAMBI to Promote Osteosarcoma Cell Migration and Invasion.
Article in Human mutation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
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4 authors.
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Abstract
Background: Distant metastasis of osteosarcoma (OS) is one of the major factors contributing to poor patient prognosis, and its underlying mechanisms remain incompletely understood. In this study, we employed single-cell RNA sequencing (scRNA-seq) to assess intratumoral heterogeneity and identify immune diagnostic and therapeutic markers, providing novel insights to guide targeted therapy. Methods: Key cell clusters were identified using InferCNV analysis and combined with differential gene expression analysis, pseudotime trajectory analysis, transcription factor regulatory network construction, and functional enrichment analysis; signaling pathways closely associated with OS metastasis were screened and subsequently validated through the GSE152048 dataset and in vitro functional experiments. Results: In this study, a total of 10 cell clusters were identified. Immune cells exhibited higher infiltration levels in the metastatic group. Further dimensionality reduction and clustering of malignant osteoblastic cells revealed five subpopulations, among which the metastatic C1 and C3 subclusters exhibited more pronounced malignant features. The results showed that BAMBI was specifically downregulated during the transition from nonmetastatic to metastatic states. Further analysis indicated that JUN may maintain subcluster phenotypic stability by regulating BAMBI expression. Moreover, as a pseudoreceptor of the TGF- Conclusion: This study reveals that during the progression of OS from a nonmetastatic to a metastatic state, BAMBI expression is downregulated and may be regulated by JUN. The decreased expression of BAMBI leads to hyperactivation of the TGF-
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