Evidence map›Paper›PMID 42211462›Full record

ArticleSeparation and purification technology2025

Separation of intrinsically magnetic cells using magnetic filters.

Jacob Strayer, Hyeon Choe, Xian Wu, Poornima Ramesh Iyer, Jenifer Gómez-Pastora, Will Moorman, Joseph Cefaratti, Alec West, Kristina Landes, Payal C Desai and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Separation and purification technology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Jacob StrayerWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Hyeon ChoeWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Xian WuWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Poornima Ramesh IyerWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Jenifer Gómez-PastoraDepartment of Chemical Engineering, Texas Tech University, Lubbock, TX, USA.
Will MoormanWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Joseph CefarattiWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Alec WestCollege of Arts and Sciences, The Ohio State University, Columbus, OH, USA.
Kristina LandesWexner Medical Center, The Ohio State University, Columbus, OH, USA.
Payal C DesaiLevine Cancer Institute, Atrium Health, Charlotte, NC, USA.
Andre F PalmerWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.
Maciej ZborowskiDepartment of Biomedical Engineering, Lerner Research Institute, Cleveland, OH, USA.
Jeffrey J ChalmersWilliam G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.

Funding

Fractionation of Aged RBCs Based on Hemoglobin ContentR01HL131720 · NHLBI · OHIO STATE UNIVERSITY · PI CHALMERS, JEFFREY JOHN, PALMER, ANDRE FRANCIS · 2017 to 2020
$2.7M
Development of single cell magnetophoresis to analyze and isolate glioma cancer stem cellsR21CA250118 · NCI · OHIO STATE UNIVERSITY · PI CHALMERS, JEFFREY JOHN, VENERE, MONICA · 2020 to 2021
$385k
NCI NIH HHS R21 CA250118NHLBI NIH HHS R01 HL131720
6 · The paper itself

Abstract

Cell separation is a common industrial, clinical, and laboratory technique that is typically accomplished via centrifugation. However other technologies for cell separation are employed when the samples need to be separated based on biological/physical properties apart from cell density. Magnetism is a technique that can be employed to separate biological entities. Cellular magnetism can be intrinsic (i.e., paramagnetic), such is the case for deoxygenated red blood cells (RBCs) and certain cancer cells that exhibit abnormal iron metabolism (e.g., glioblastoma), or this magnetism can be artificially induced based on magnetic particle labeling. In this work, we investigate magnetic filtration using commercial, high gradient magnetic separation (HGMS) packed columns for the separation of intrinsically magnetic RBCs. Specifically, flow rate, cell concentration, and external field strength were investigated using Miltenyi LS columns as magnetic filters. A generalized model for magnetic filtration was applied and used to investigate potential scale up of our RBC magnetic filtration process. COMSOL Multiphysics numerical simulations were performed to quantify the effect of the external field strength on the local magnetic energy gradient generated inside the ferromagnetic packed column. It was found that varying the externally supplied magnetic field from 0.1 to 1 T produces a non-linear increase in the magnetic energy gradient local to the ferromagnetic packing structure in the separator. Finally, the magnetic separator was used to demonstrate the capability of a binary separation of an artificially produced mixture of methemoglobin (metHb) containing RBCs (paramagnetic) from non-oxidized ferrous oxyhemoglobin (oxyHb) containing RBCs (diamagnetic). It was shown that 99 % of the magnetically isolated cells had a magnetic signature indicative of metHb-RBCs. In conclusion, we demonstrated the application of a magnetic filtration model for RBC separation and its utility to study the process variable effects on the separation's performance.

Indexed as

Computational simulationHigh gradient magnetic separationMagnetic separationNumerical modelingPacked column separationRed blood cells

Identifiers

PMID42211462
PMCPMC13215711

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.