ArticleSurgery open science2026
Non-invasive
Article in Surgery open science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Xenotransplantation of pluripotent stem cell-derived human intestinal organoids (HIOs) into animal hosts models early human small intestinal development. However, traditional methods to assess HIO engraftment and expansion involve invasive, non-survival procedures. We developed a novel HIO platform featuring stable intestinal epithelial expression of a red-shifted luciferase, enabling sensitive, longitudinal Methods: Human embryonic stem cell (hESC) line H9 was lentivirus-transduced with a plasmid encoding red-shifted luciferase under a ubiquitin C promoter. After positive selection, cells were differentiated into endoderm, mid/hindgut spheroids, and HIOs. HIO maturation and luciferase expression were confirmed by immunofluorescence prior to kidney capsule implantation in 10 adult immunodeficient SCID mice. We performed serial Results: Stable transduction did not compromise the genome integrity of hESCs, pluripotency, or maturation of hESCs into HIOs. In 9 of 10 mice, HIO radiance was detectable within two weeks of implantation. Mean radiance significantly increased over time ( Conclusions: We successfully generated transplantable bioluminescent HIOs from pluripotent stem cells, setting the stage for dynamic monitoring of HIOs to further elucidate human intestinal development and injury response across the lifespan and other diverse applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.