Evidence map›Paper›PMID 42210913›Full record

ArticleChemistry (Weinheim an der Bergstrasse, Germany)2026

Covalent Insertion of a Mn(Salen) Type Complex in Cross-Linked Protein Crystals: Design of an Enantioselective Artificial Epoxidase.

Manel Boukhallat, Ismail Benhamed, Jade Arnone, Stann Van Baaren, Clara Rinaldi, Patrice Catty, Caroline Marchi-Delapierre, Christine Cavazza, Stéphane Ménage

Abstract read
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In one paragraph

Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Manel BoukhallatUniv. Grenoble-Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, BioCE team, Grenoble, France.
Ismail BenhamedUniv. Grenoble-Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, BioCE team, Grenoble, France.
Jade ArnoneUniv. Grenoble-Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, BioCE team, Grenoble, France.
Stann Van BaarenUniv. Grenoble-Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, BioCE team, Grenoble, France.
Clara RinaldiUniv. Grenoble. Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, Grenoble, France.
Patrice CattyUniv. Grenoble. Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, Grenoble, France.
Caroline Marchi-DelapierreUniv. Grenoble. Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, Grenoble, France.
Christine CavazzaUniv. Grenoble. Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, Grenoble, France.ORCID https://orcid.org/0000-0002-3657-1302
Stéphane MénageUniv. Grenoble-Alpes, CNRS, CEA, IRIG, Laboratoire de Chimie et Biologie des Métaux, BioCE team, Grenoble, France.ORCID https://orcid.org/0000-0003-4512-8836

Funding

Agence Nationale de la Recherche ANR-18-CE07-0034-2PRCNI(k)AGARAGraduate School on Chemistry, Biology and Health of Univ Grenoble Alpes CBH-EUR-GS ANR-17-EURE-0003LabEx ARCANE ANR-11-LABX-0003-01
6 · The paper itself

Abstract

Artificial enzymes represent a promising alternative for performing non-natural reactions in biocatalysis. Here, we illustrate the potential of cross-linked enzyme crystals (CLEC) to achieve enantioselective epoxidation through the generation of an artificial enzyme obtained by direct covalent anchoring of a manganese complex as an artificial active site within a protein. Enantiomeric excess (ee) of up to 90% on cis-β-methylstyrene was measured when the covalent binding yield was maximized, thanks to the remarkable behavior of the crystals. The structure of the modified enzyme, NikA, is provided. This work adds to the growing body of examples highlighting the advantages of CLEC in oxidation catalysis.

Indexed as

Epoxy CompoundsEthylenediaminesManganeseBiocatalysisCatalytic DomainCross-Linking ReagentsCrystallography, X-RayModels, MolecularOxidation-ReductionStereoisomerismCross-Linking Reagentsdisalicylaldehyde ethylenediamineEpoxy CompoundsEthylenediaminesManganeseartificial enzymesbioconjugationcross‐linked enzyme crystalsdirected synthesisepoxidationmanganese

Identifiers

PMID42210913
PMCPMC13569931

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.