ArticleJournal of integrative plant biology2026
Magnetic nanoparticle-mediated genetic transformation and gene editing system in loquat (Eriobotrya japonica).
Article in Journal of integrative plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Magnetic nanoparticle-mediated genetic transformation and gene editing system in loquat (Eriobotrya japonica).Journal of integrative plant biology · 2026Article
- Unlocking the power and potential of the Cas12 family members and their ancestors for crop genome editing.Journal of integrative plant biology · 2026Article
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12 authors.
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Abstract
Loquat (Eriobotrya japonica Lindl.) is a valuable subtropical fruit tree whose genetic improvement has been significantly constrained by the absence of an efficient genetic transformation system. Although Agrobacterium-mediated transformation is the most widely used method, it proves ineffective in loquat due to the species' recalcitrance to in vitro regeneration. Pollen-based transformation offers a promising alternative by bypassing the need for tissue culture. However, the pollen wall poses a major physical barrier to the uptake of exogenous DNA. In this study, we investigated magnetic nanoparticle (MNP)-mediated transformation as a novel strategy for loquat. We confirmed that loquat pollen contains tricolporate apertures with diameters ranging from 3.0 to 5.0 μm, which are structurally suitable for the entry of MNPs-DNA. Based on this finding, we developed and optimized a transformation protocol using polyethyleneimine-coated Fe
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